Stingless bees are endemic in northeastern Brazil, where they are exploited for honey production and crop pollination. The therapeutic use of honey can be attributed to its antibacterial and antioxidant activities. The effects of time and storage temperature on Melipona scutellaris honey were evaluated by physicochemical and microbiological parameters as well as by the analysis of bioactive compounds and antioxidant activity. Fresh honey samples were monitored at 5 and 30 °C for 150 days. The total acidity and HMF increased, while pH and diastase activity significantly decreased over time at 30 °C (p < 0.05). Fresh honey met all the established parameters. In contrast, due to storage, it failed to meet the criteria set in the current legislation for Melipona with the most drastic shortcomings observed at 30 °C. Unlike monosaccharides, acetic and lactic acid concentrations increased, indicating fermentation. During storage, the total phenolic compounds and flavonoids contents were reduced along with antioxidant activities measured by DPPH, ABTS, and FRAP methods. On the 60th day of storage at 5 °C, dextrose crystallization began, and the water content increased. Storage at 5 °C for up to 60 days resulted in minor changes, but after 150 days, storage at 30 °C showed reduced bioactive degradation and higher antioxidant activity. Therefore, the storage conditions associated with crystallization were found to control the stability of Melipona scutellaris honey.

无刺蜜蜂在巴西东北部很流行，在那里它们被用于生产蜂蜜和为作物授粉。蜂蜜的治疗用途可归因于其抗菌和抗氧化活性。通过理化和微生物参数以及生物活性化合物和抗氧化活性分析，评估了时间和储存温度对黄芩蜂蜜的影响。新鲜蜂蜜样品在 5 和 30 °C 下监测 150 天。在 30 °C 时，总酸度和 HMF 增加，而 pH 和淀粉酶活性随时间显着降低 (p  <  0.05)。新鲜蜂蜜符合所有既定参数。相比之下，由于存储，它未能满足现行立法中规定的标准Melipona在 30 °C 时观察到最严重的缺点 。与单糖不同，乙酸和乳酸浓度增加，表明发酵。在储存期间，总酚类化合物和类黄酮含量随着 DPPH、ABTS 和 FRAP 方法测量的抗氧化活性而降低。5℃保存第60天 ，葡萄糖开始结晶，水分增加。在 5 °C 下储存 长达 60 天会产生微小变化，但在 150 天后，在 30 °C 下储存 会减少生物活性降解并提高抗氧化活性。因此，发现与结晶相关的储存条件可以控制黄芩蜂蜜的稳定性。