Non-small cell lung cancer (NSCLC) is the most common subtype of lung cancer, and it is characterized by a high incidence. It is important to understand the molecular mechanisms that determine the progression and metastasis of NSCLC in order to develop more effective therapies and identify novel diagnostic indicators of NSCLC. RSPH14 has been reported to be related to multiple human diseases, including duodenal adenocarcinoma and meningiomas, but the role of RSPH14 in NSCLC remains unclear. The present study aimed to investigate the molecular function and clinical significance of RSPH14 in NSCLC. Analyses of public datasets and clinical samples demonstrated that RSPH14 expression was upregulated in NSCLC samples compared with normal samples. In addition, high RSPH14 expression was associated with a shorter overall survival time in patients with NSCLC. Notably, RSPH14 knockdown suppressed the proliferation and cell cycle progression and enhanced the apoptosis of NSCLC cells. Mechanically, tandem mass tag analysis demonstrated that RSPH14 can affect multiple processes, including the AMPK signaling pathway, calcium ion import regulation, glucose transmembrane transporter activity, and glucose transmembrane transport. Taken together, the results of the present study suggest that RSPH14 may be a promising prognostic factor and therapeutic target for NSCLC.

中文翻译：

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RSPH14调节非小细胞肺癌细胞的增殖、细胞周期进程和凋亡

非小细胞肺癌（NSCLC）是肺癌最常见的亚型，具有发病率高的特点。了解决定 NSCLC 进展和转移的分子机制对于开发更有效的治疗方法并确定 NSCLC 的新诊断指标非常重要。据报道，RSPH14 与多种人类疾病有关，包括十二指肠腺癌和脑膜瘤，但 RSPH14 在 NSCLC 中的作用仍不清楚。本研究旨在探讨RSPH14在NSCLC中的分子功能及临床意义。对公共数据集和临床样本的分析表明，与正常样本相比，非小细胞肺癌样本中的 RSPH14 表达上调。此外，高 RSPH14 表达与 NSCLC 患者较短的总生存期相关。值得注意的是，RSPH14 敲低抑制了 NSCLC 细胞的增殖和细胞周期进程，并增强了细胞凋亡。机械上，串联质量标签分析表明，RSPH14 可以影响多个过程，包括 AMPK 信号通路、钙离子输入调节、葡萄糖跨膜转运蛋白活性和葡萄糖跨膜转运。总之，本研究的结果表明，RSPH14 可能是 NSCLC 的一个有前途的预后因素和治疗靶点。包括AMPK信号通路、钙离子输入调控、葡萄糖跨膜转运蛋白活性和葡萄糖跨膜转运。总之，本研究的结果表明，RSPH14 可能是 NSCLC 的一个有前途的预后因素和治疗靶点。包括AMPK信号通路、钙离子输入调控、葡萄糖跨膜转运蛋白活性和葡萄糖跨膜转运。总之，本研究的结果表明，RSPH14 可能是 NSCLC 的一个有前途的预后因素和治疗靶点。