The health-promoting effects along with global economic importance of consuming food products supplemented with probiotic microorganisms encouraged the researchers to discover new probiotics. Fourteen lactic acid bacterial isolates were identified as Enterococcus mediterraneensis, Lactobacillus fermentum, and Streptococcus lutetiensis by 16S rRNA gene sequencing, and in vitro characterized for their actual probiotic potential. All E. mediterraneensis isolates were resistant to clindamycin, whereas Lb. fermentum isolates were resistant to ampicillin, clindamycin, and vancomycin. The E. mediterraneensis and Lb. fermentum isolates displayed high overall digestive survival, ranged from 1.35 ± 0.06 to 32.73 ± 0.84% and from 2.01 ± 0.01 to 23.9 ± 1.85%, respectively. All isolates displayed cell surface hydrophobicity, ranged between 15.44 ± 6.72 and 39.79 ± 2.87%. The strongest auto-aggregation capability, higher than 40%, was observed for most E. mediterraneensis and Lb. fermentum isolates. The E. mediterraneensis isolates (L2, L12, and L15), Lb. fermentum (L8, L9, and L10), and Strep. lutetiensis (L14) exhibited the greatest co-aggregation with Salmonella typhimurium, Escherichia coli O157:H7, Staphylococcus aureus, and Bacillus cereus. Fifty-seven and fourteen hundredth percent of E. mediterraneensis isolates could be considered bacteriocinogenic against E. coli O157:H7, B. cereus, and S. aureus. This study is the first one to isolate Enterococcus mediterraneensis in Egypt and to characterize it as new species of probiotics globally. According to the results, E. mediterraneensis (L2, L12, and L15), Lb. fermentum (L8, L9, and L10), and Strep. lutetiensis (L14) are the most promising in vitro probiotic candidates.

中文翻译：

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一些埃及乳酸菌和酵母菌益生菌功能特性的分子鉴定和体外评价

食用补充有益生菌微生物的食品对健康的促进作用以及全球经济重要性鼓励研究人员发现新的益生菌。通过 16S rRNA 基因测序，14 株乳酸菌被鉴定为地中海肠球菌、发酵乳杆菌和黄体链球菌，并在体外对其实际益生菌潜力进行了表征。所有 E. mediterraneensis 分离株都对克林霉素具有抗性，而 Lb。发酵菌株对氨苄青霉素、克林霉素和万古霉素耐药。E. mediterraneensis 和 Lb。发酵菌株显示出较高的总体消化存活率，范围分别为 1.35 ± 0.06 至 32.73 ± 0.84% 和 2.01 ± 0.01 至 23.9 ± 1.85%。所有分离株都显示出细胞表面疏水性，范围在 15.44 ± 6.72 和 39 之间。79±2.87%。对于大多数 E. mediterraneensis 和 Lb，观察到最强的自动聚集能力，高于 40%。发酵分离物。E. mediterraneensis 分离株（L2、L12 和 L15）、Lb。发酵菌（L8、L9 和 L10）和链球菌。lutetiensis (L14) 与鼠伤寒沙门氏菌、大肠杆菌O157:H7、金黄色葡萄球菌和蜡状芽孢杆菌的共聚集表现最大。57% 和 14% 的 E. mediterraneensis 分离株可被认为对 E.coli O157:H7、B. cereus 和 S. aureus 具有杀菌作用。这项研究是第一个在埃及分离出地中海肠球菌并将其定性为全球益生菌新物种的研究。根据结果​​，E. mediterraneensis（L2、L12 和 L15）、Lb。发酵菌（L8、L9 和 L10）和链球菌。