当前位置: X-MOL 学术Dev. Comp. Immunol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Response to Bombyx mori nucleopolyhedrovirus infection in silkworm: Gut metabolites and microbiota
Developmental & Comparative Immunology ( IF 2.9 ) Pub Date : 2021-08-04 , DOI: 10.1016/j.dci.2021.104227
Xiu Shi 1 , Yaxin Zhang 1 , Tianchen Zhu 1 , Nan Li 1 , Sufei Sun 1 , Min Zhu 1 , Jun Pan 1 , Zeen Shen 1 , Xiaolong Hu 2 , Xing Zhang 1 , Chengliang Gong 2
Affiliation  

The diversity of microbiota and metabolites in the digestive tract gut is important in physiology and homeostasis, nutrient uptake and virus infection. In lepidopteran insect model silkworms, little is known about how microbiota and metabolites are altered after oral infection with BmNPV. Herein, we used 16S rDNA sequencing and metabolomics to show that the gut microbiota and metabolites of silkworm midgut are significantly altered after BmNPV infection. Kyoto Encyclopedia of Genes and Genomes analysis revealed enrichment of flavone and flavonol biosynthesis, glycosyltransferases, and electron transfer carriers signaling pathways, suggesting potential roles in viral infection. Infection also changed the abundance of metabolites in the digestive tract gut, where most pathways were related to metabolism of amino acids, fatty acids and other pathways (e.g., citrate cycle). In addition, a correlation was observed between digestive tract gut microbiota and metabolites. These results shed light on the interaction between digestive tract gut microbiota, metabolites and host-virus interaction, and enhance our understanding of viral infection links to midgut microbiota and metabolic activity in the silkworm.



中文翻译:

家蚕对家蚕核型多角体病毒感染的反应:肠道代谢物和微生物群

消化道肠道中微生物群和代谢物的多样性对生理和体内平衡、营养吸收和病毒感染很重要。在鳞翅目昆虫模型蚕中,人们对口腔感染 BmNPV 后微生物群和代谢物如何改变知之甚少。在此,我们使用 16S rDNA 测序和代谢组学表明家蚕中肠的肠道微生物群和代谢物在 BmNPV 感染后发生了显着变化。京都基因百科全书和基因组分析揭示了黄酮和黄酮醇生物合成、糖基转移酶和电子转移载体信号通路的富集,表明在病毒感染中的潜在作用。感染还改变了消化道肠道中代谢物的丰度,其中大多数途径与氨基酸代谢有关,脂肪酸和其他途径(例如,柠檬酸循环)。此外,在消化道肠道微生物群和代谢物之间观察到相关性。这些结果揭示了消化道肠道微生物群、代谢物和宿主-病毒相互作用之间的相互作用,并增强了我们对病毒感染与家蚕中肠微生物群和代谢活动之间联系的理解。

更新日期:2021-08-07
down
wechat
bug