Concentrated growth factor (CGF) membranes are widely used in basic and clinical research of soft and hard tissue regeneration, but its effect on periodontal tissue regeneration is less studied. This study explored the role of CGF membranes in periodontal tissue regeneration mediated by human umbilical cord mesenchymal stem cells (hUCMSCs). HUCMSCs and human periodontal ligament fibroblasts (HPLFs) were extracted and identified by microscope and flow cytometry. The effects of the extracted CGF membrane on cell viability, osteogenic differentiation ability, osteopontin (OPN) expression, alkaline phosphatase (ALP) content, and osteogenic differentiation-related genes (Runt-related transcription factor 2 (RUNX2); osteocalcin (OCN); ALP), Tafazzin (TAZ) expression, and nuclear transfer were examined by MTT assay, alizarin red staining, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. Rescue experiments were performed to examine the effects of TAZ transfection and cell coculture. In the identified hUCMSCs (positive expressions of CD29, CD44, CD146, and CD105), overexpressed TAZ (pc-TAZ) enhanced the promotive effect of CGF membrane on cell viability, cell cycle, mineralization, ALP content and expressions of OPN, TAZ and osteogenic differentiation-related genes, and nuclear transfer. However, silencing TAZ showed opposite effects. The coculture of hUCMSCs and HPLFs further promoted the basic biological functions of HPLFs by upregulating osteogenic differentiation-related genes and COL-1 but downregulated MMP1 expression. Pc-TAZ could enhance the effect of CGF membrane on promoting periodontal tissue regeneration. CGF membrane promoted periodontal tissue regeneration through upregulating TAZ and osteogenic differentiation-related genes.

中文翻译：

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CGF 膜通过上调 TAZ 和成骨分化基因促进 hUCMSCs 介导的牙周组织再生

浓缩生长因子（CGF）膜广泛应用于软硬组织再生的基础和临床研究，但其对牙周组织再生的影响研究较少。本研究探讨了 CGF 膜在人脐带间充质干细胞 (hUCMSCs) 介导的牙周组织再生中的作用。提取 HUCMSCs 和人牙周膜成纤维细胞 (HPLFs) 并通过显微镜和流式细胞术进行鉴定。提取的CGF膜对细胞活力、成骨分化能力、骨桥蛋白（OPN）表达、碱性磷酸酶（ALP）含量和成骨分化相关基因（Runt相关转录因子2（RUNX2）；骨钙素（OCN）； ALP)、Tafazzin (TAZ) 表达和核转移通过 MTT 测定、茜素红染色、免疫荧光、酶联免疫吸附测定 (ELISA)、定量实时聚合酶链反应 (qRT-PCR) 和蛋白质印迹。进行拯救实验以检查 TAZ 转染和细胞共培养的效果。在鉴定的 hUCMSCs（CD29、CD44、CD146 和 CD105 阳性表达）中，过表达的 TAZ（pc-TAZ）增强了 CGF 膜对细胞活力、细胞周期、矿化、ALP 含量和 OPN、TAZ 和成骨分化相关基因和核转移。然而，沉默 TAZ 显示出相反的效果。hUCMSCs和HPLFs的共培养通过上调成骨分化相关基因和COL-1但下调MMP1的表达，进一步促进了HPLFs的基本生物学功能。Pc-TAZ可以增强CGF膜促进牙周组织再生的作用。CGF 膜通过上调 TAZ 和成骨分化相关基因促进牙周组织再生。