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Changing the shape of wool yarns via laccase-mediated grafting of tyrosine
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2021-08-05 , DOI: 10.1016/j.jbiotec.2021.08.001
Yu Li 1 , Jennifer Noro 2 , Madalena Martins 2 , Su Jing 1 , Carla Silva 2 , Artur Cavaco-Paulo 3
Affiliation  

The shape of wool yarns was changed by laccase-assisted grafting of tyrosine. Prior to tyrosine grafting a cysteine pre-treatment was optimized aiming to increase the amount of thiol reaction groups available. The best operational conditions for laccase-assisted tyrosine grafting were: i) pre-treatment with cysteine (2.2 mM) in a solution of 20 % ethanol, 15 % propylene glycol and 0.5 % benzyl alcohol, pH = 10, 40 °C; ii) tyrosine grafting with 3.0 mM tyrosine, 18 U/mL laccase, pH = 5, 40 °C. The shape modification was evaluated by number of curly twists determination on the grafted yarn samples. The thermal and mechanical properties of the grafted wool yarns was evaluated by TGA, DSC and breaking strength determination. The amount of free thiols and weight gain were assessed aiming to infer the role of the cysteine pre-treatment on the final tyrosine grafting and shape modification. The laccase-assisted grafting of tyrosine onto wool yarns have influenced the thermal and mechanical properties of the yarns however without compromising their structural integrity for the final application purposes. The developed methodology to impart new shape to wool yarns is presented herein as an environmentally friendly alternative to chemical methods. The new findings revealed great potentialities for application in similar fibers like hair.



中文翻译:

通过漆酶介导的酪氨酸接枝改变毛纱的形状

通过漆酶辅助酪氨酸接枝改变毛纱的形状。在酪氨酸接枝之前,对半胱氨酸预处理进行了优化,旨在增加可用的硫醇反应基团的数量。漆酶辅助酪氨酸接枝的最佳操作条件是:i) 在 20% 乙醇、15% 丙二醇和 0.5% 苯甲醇的溶液中用半胱氨酸 (2.2 mM) 预处理,pH = 10,40 °C;ii) 酪氨酸与 3.0 mM 酪氨酸、18 U/mL 漆酶、pH = 5、40 °C 的接枝。通过对接枝纱线样品的卷曲捻数测定来评估形状改变。通过TGA、DSC和断裂强度测定来评价接枝毛纱的热性能和机械性能。评估游离硫醇的量和体重增加,旨在推断半胱氨酸预处理对最终酪氨酸接枝和形状修饰的作用。漆酶辅助将酪氨酸接枝到羊毛纱线上已经影响了纱线的热性能和机械性能,但不会影响其最终应用目的的结构完整性。本文介绍了赋予羊毛纱线新形状的开发方法,作为化学方法的一种环保替代方法。新发现揭示了在头发等类似纤维中应用的巨大潜力。漆酶辅助将酪氨酸接枝到羊毛纱线上已经影响了纱线的热性能和机械性能,但不会影响其最终应用目的的结构完整性。本文介绍了赋予羊毛纱线新形状的开发方法,作为化学方法的一种环保替代方法。新发现揭示了在头发等类似纤维中应用的巨大潜力。漆酶辅助将酪氨酸接枝到羊毛纱线上已经影响了纱线的热性能和机械性能,但不会影响其最终应用目的的结构完整性。本文介绍了赋予羊毛纱线新形状的开发方法,作为化学方法的一种环保替代方法。新发现揭示了在头发等类似纤维中应用的巨大潜力。

更新日期:2021-08-15
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