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Inactivation of Staphylococcus aureus and Escherichia coli Biofilms by Air-Based Atmospheric-Pressure DBD Plasma
Applied Biochemistry and Biotechnology ( IF 3 ) Pub Date : 2021-08-04 , DOI: 10.1007/s12010-021-03636-3
S Khosravi 1 , S Jafari 2 , H Zamani 3 , M Nilkar 2, 4
Affiliation  

Air-based atmospheric-pressure plasma is an effective non-thermal method in deactivating various kinds of microbial biofilms with several advantages, including high bactericidal efficiency and low treatment costs. Bacterial biofilm formation is a major determinant in establishment of bacterial infection and also resistance to antibacterial chemotherapy. This study aims to assess the anti-biofilm potential of air-based atmospheric-pressure DBD plasma against Staphylococcus aureus and Escherichia coli biofilms. The biofilms of Staphylococcus aureus and Escherichia coli were exposed to air-based atmospheric-pressure DBD plasma for up to 4 min (control, 30 s, 90 s, 3 min, and 4 min) and their biofilm formation level, viability, and membrane integrity were determined. Based on the results, plasma exposure caused disruption up to 70% and 85% for S. aureus and E. coli biofilms, respectively. The biofilm disruption potential of air-based atmospheric-pressure DBD plasma was confirmed using the scanning electron microscopy (SEM). Besides, based on confocal laser scanning microscopy (CLSM), plasma exposure caused a significant bacterial inactivation and E. coli was found as more susceptible strain than S. aureus. In conclusion, atmospheric-pressure DBD plasma could be considered an efficient non-thermal approach against bacterial pathogenicity by biofilm disruption and thus prevention of infection establishment.



中文翻译:

气压 DBD 等离子体灭活金黄色葡萄球菌和大肠杆菌生物膜

基于空气的大气压等离子体是灭活各种微生物生物膜的一种有效的非热方法,具有杀菌效率高和处理成本低等优点。细菌生物膜的形成是细菌感染的主要决定因素,也是对抗菌化学疗法的抗性。本研究旨在评估基于空气的大气压 DBD 等离子体对金黄色葡萄球菌大肠杆菌生物膜的抗生物膜潜力。金黄色葡萄球菌大肠杆菌的生物膜暴露于基于空气的大气压 DBD 等离子体长达 4 分钟(对照、30 秒、90 秒、3 分钟和 4 分钟),并确定它们的生物膜形成水平、活力和膜完整性。根据结果​​,血浆暴露对金黄色葡萄球菌大肠杆菌生物膜分别造成高达 70% 和 85% 的破坏。使用扫描电子显微镜 (SEM) 证实了基于空气的大气压 DBD 等离子体的生物膜破坏潜力。此外,基于共聚焦激光扫描显微镜(CLSM),等离子体暴露导致显着的细菌灭活,发现大肠杆菌金黄色葡萄球菌更敏感。 总之,大气压 DBD 等离子体可以被认为是一种有效的非热方法,通过生物膜破坏来对抗细菌致病性,从而防止感染建立。

更新日期:2021-08-10
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