Our study was aimed to investigate the effects of lgals3a (Gal-3 encoding gene) on the development of zebrafish embryo and its underlying mechanisms. Morpholino (MO) technology was used to inhibit the expression of zebrafish lgals3a, and the effect of lgals3a gene knockdown on zebrafish embryo development and the number of monocyte macrophages was observed. Effect of lgals3a-e3i3-MO on apoptosis of zebrafish was detected by acridine orange staining. In addition, the mRNA expression levels of Wnt/β-catenin signaling pathway-related genes were detected by RT-qPCR. Compared with control-MO group, the zebrafish embryos injected with lgals3a-e3i3-MO had obvious defects in the head, eyes, and tail, and pericardial edema. Lgals3a-e3i3-MO significantly reduced the number of mononuclear macrophages in zebrafish embryos compared with the control-MO group. The results of acridine orange staining showed that compared with the control-MO group, lgals3a-e3i3-MO promoted cardiomyocyte apoptosis in zebrafish. Furthermore, lgals3a-e3i3-MO significantly up-regulated the expression of dkk1b, wnt9a, lrp5, fzd7a, β-catenin, Gsk-3β, mycn, myca in the Wnt/β-catenin pathway, and decreased the expression of lef1. These results indicate that lgals3a-e3i3-MO inhibits zebrafish embryo development, reduces the number of mononuclear macrophages, activates Wnt/β-catenin signaling pathway and promotes cardiomyocyte apoptosis.

我们的研究旨在研究lgals3a（Gal-3编码基因）对斑马鱼胚胎发育的影响及其潜在机制。采用吗啉代(MO)技术抑制斑马鱼lgals3a的表达，观察敲除lgals3a基因对斑马鱼胚胎发育和单核巨噬细胞数量的影响。吖啶橙染色检测lgals3a-e3i3-MO对斑马鱼凋亡的影响。此外，通过RT-qPCR检测Wnt/β-catenin信号通路相关基因的mRNA表达水平。与对照-MO组相比，注射lgals3a-e3i3-MO的斑马鱼胚胎头部、眼睛和尾部有明显缺陷，心包水肿。与对照组相比，Lgals3a-e3i3-MO 显着减少了斑马鱼胚胎中单核巨噬细胞的数量。吖啶橙染色结果表明，与对照-MO组相比，lgals3a-e3i3-MO促进斑马鱼心肌细胞凋亡。此外，lgals3a-e3i3-MO显着上调Wnt/β-catenin通路中dkk1b、wnt9a、lrp5、fzd7a、β-catenin、Gsk-3β、mycn、myca的表达，并降低lef1的表达。这些结果表明lgals3a-e3i3-MO抑制斑马鱼胚胎发育，减少单核巨噬细胞数量，激活Wnt/β-catenin信号通路，促进心肌细胞凋亡。myca 在 Wnt/β-catenin 通路中，并降低 lef1 的表达。这些结果表明lgals3a-e3i3-MO抑制斑马鱼胚胎发育，减少单核巨噬细胞数量，激活Wnt/β-catenin信号通路，促进心肌细胞凋亡。myca 在 Wnt/β-catenin 通路中，并降低 lef1 的表达。这些结果表明lgals3a-e3i3-MO抑制斑马鱼胚胎发育，减少单核巨噬细胞数量，激活Wnt/β-catenin信号通路，促进心肌细胞凋亡。