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The Genome of the Haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales): A Model for Lipid Biosynthesis in Eukaryotic Algae
Genome Biology and Evolution ( IF 3.3 ) Pub Date : 2021-07-30 , DOI: 10.1093/gbe/evab178
Chris J Hulatt 1, 2 , René H Wijffels 1, 3 , Matthew C Posewitz 2
Affiliation  

Haptophytes are biogeochemically and industrially important protists with underexplored genomic diversity. We present a nuclear genome assembly for the class Pavlovales, which was assembled with PacBio long-read data into highly contiguous sequences. We sequenced strain Diacronema lutheri NIVA-4/92, formerly known as Pavlova lutheri, because it has established roles in aquaculture and has been a key organism for studying microalgal lipid biosynthesis. Our data show that D. lutheri has the smallest and most streamlined haptophycean genome assembled to date, with an assembly size of 43.503 Mb and 14,446 protein-coding genes. Together with its high nuclear GC content, Diacronema is an important genus for investigating selective pressures on haptophyte genome evolution, contrasting with the much larger and more repetitive genome of the coccolithophore Emiliania huxleyi. The D. lutheri genome will be a valuable resource for resolving the genetic basis of algal lipid biosynthesis and metabolic remodeling that takes place during adaptation and stress response in natural and engineered environments.

中文翻译:

Haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales) 的基因组:真核藻类脂质生物合成的模型

触体是生物地球化学和工业上重要的原生生物,其基因组多样性尚未得到充分探索。我们提出了巴甫洛瓦目类的核基因组组装,它与 PacBio 长读数据组装成高度连续的序列。我们对菌株 Diacronema lutheri NIVA-4/92(以前称为 Pavlova lutheri)进行了测序,因为它已在水产养殖中确立了作用,并且一直是研究微藻脂质生物合成的关键生物。我们的数据显示,D. lutheri 具有迄今为止组装的最小和最精简的触藻基因组,组装大小为 43.503 Mb 和 14,446 个蛋白质编码基因。连同其高核 GC 含量,Diacronema 是研究对 haptophyte 基因组进化的选择压力的重要属,与球石藻 Emiliania huxleyi 更大、更重复的基因组形成对比。D. lutheri 基因组将成为解决藻类脂质生物合成和代谢重塑的遗传基础的宝贵资源,这些遗传基础发生在自然和工程环境中的适应和应激反应过程中。
更新日期:2021-07-30
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