The emerging community of cell-free synthetic biology aspires to build complex biochemical and genetic systems with functions that mimic or even exceed those in living cells. To achieve such functions, cell-free systems must be able to sense and respond to the complex chemical signals within and outside the system. Cell-free riboswitches can detect chemical signals via RNA–ligand interaction and respond by regulating protein synthesis in cell-free protein synthesis systems. In this article, we review synthetic cell-free riboswitches that function in both prokaryotic and eukaryotic cell-free systems reported to date to provide a current perspective on the state of cell-free riboswitch technologies and their limitations.

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无细胞核糖开关

新兴的无细胞合成生物学社区渴望建立复杂的生化和遗传系统，其功能模仿甚至超越活细胞。为了实现这些功能，无细胞系统必须能够感知和响应系统内外的复杂化学信号。无细胞核糖开关可以通过RNA-配体相互作用检测化学信号，并通过调节无细胞蛋白质合成系统中的蛋白质合成来做出反应。在本文中，我们回顾了迄今为止报道的在原核和真核无细胞系统中均起作用的合成无细胞核糖开关，以提供有关无细胞核糖开关技术状态及其局限性的当前观点。