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Erratum
Journal of Cellular Biochemistry ( IF 4 ) Pub Date : 2021-08-03 , DOI: 10.1002/jcb.30115


Unfortunately,

in the article “Evidence for phosphatase activity of p27SJ and its impact on the cell cycle” by Nune et al. published in the Journal of Cellular Biochemistry 107:400-407 (2009),1 Figure 4, its legend and the corresponding text had errors. The revised figure and the text describing Figure 4C on page 404 are as follows.

image
Figure 4
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Effect of p27SJ on cyclin A and cell cycle progression. (A) Western blot showing the level of cyclin A, Cdk2, and tubulin in the absence (Dox−) or presence (Dox+) of p27SJ. (B) H1 kinase assay of immunocomplex containing cyclin A (lanes 1 and 2) or the complex pulled down by control normal serum (lanes 3 and 4) from U-87MG extract before (lanes 1 and 3) and after (lanes 2 and 4) treatment with Dox. (C) Cell cycle analysis of U-87MG before (−p27SJ) and after (+p27SJ) treatment with Dox at various times (0, 16, 24, and 28 h) after release from serum starvation. The numbers represent the average of two experiments

The correct text describing Figure 4C, section “Results and Discussion,” p. 404 should read:

As seen in Figure 4C, In the absence of p27SJ (no doxycycline treatment), cells progressed from G0/G1 to S at 24 h and to G2/M at 28 h after release from serum starvation. In cells expressing p27SJ, cells also progressed from G0/G1 to S phase at 24 h with approximately 8% decreased levels compared with those from p27SJ-negative cells. Also, progression of the cells from S to G2/M was reduced in p27SJ cells. Unlike the control cells, which had 27.4% of the cells at G2/M stage, only 16.5% of the cells expressing p27SJ at 28 h were detected at the G2/M phase. These results taken together show that dysregulation of cell cycle factors including cyclin A, which was shown to impact S and G2 phases (Pagano et al., 1992), has a biological consequence on cell cycle progression of the p27SJ-expressing cells.



中文翻译:

勘误表

很遗憾,

在 Nune 等人的文章“p27SJ 磷酸酶活性的证据及其对细胞周期的影响”中。发表于Journal of Cellular Biochemistry 107:400-407 (2009),1图4,其图例及对应文字有错误。修改后的图和描述第 404 页图 4C 的文字如下。

图片
图 4
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p27SJ 对细胞周期蛋白 A 和细胞周期进程的影响。(A) 蛋白质印迹显示 p27SJ 不存在 (Dox-) 或存在 (Dox+) 时细胞周期蛋白 A、Cdk2 和微管蛋白的水平。(B) 含有细胞周期蛋白 A 的免疫复合物(泳道 1 和 2)或复合物的 H1 激酶测定,该复合物被来自 U-87MG 提取物的对照正常血清(泳道 3 和 4)之前(泳道 1 和 3)和之后(泳道 2 和4)用Dox处理。(C) 在从血清饥饿释放后的不同时间(0、16、24 和 28 小时)用 Dox 处理之前(-p27SJ)和之后(+p27SJ)的 U-87MG 的细胞周期分析。数字代表两次实验的平均值

描述图 4C,“结果和讨论”部分的正确文本,p。404 应该是:

如图 4C 所示,在没有 p27SJ(无强力霉素处理)的情况下,细胞在 24 小时从 G0/G1 进展到 S,在从血清饥饿释放后的 28 小时进展到 G2/M。在表达 p27SJ 的细胞中,细胞也在 24 小时从 G0/G1 进展到 S 期,与来自 p27SJ 阴性细胞的细胞相比,水平降低了约 8%。此外,在 p27SJ 细胞中,细胞从 S 到 G2/M 的进展减少。与在 G2/M 阶段具有 27.4% 细胞的对照细胞不同,在 G2/M 阶段仅检测到 16.5% 在 28 小时时表达 p27SJ 的细胞。这些结果综合起来表明,包括细胞周期蛋白 A 在内的细胞周期因子失调,其已被证明会影响 S 和 G2 期(Pagano 等,1992),对表达 p27SJ 的细胞的细胞周期进程具有生物学影响。

更新日期:2021-09-27
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