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Genome-Wide Expression Difference of MicroRNAs in Basal Cell Carcinoma
Journal of Immunology Research ( IF 4.1 ) Pub Date : 2021-08-04 , DOI: 10.1155/2021/7223500 Hai-Peng Wei 1 , Song Zhan 1 , Qing-An Zhu 1 , Zhen-Juan Chen 1 , Xian Feng 1 , Jun-Yuan Chen 2 , Qi-Lin Zhang 1, 3 , Jingjie Zhao 4 , Lingzhang Meng 5
Journal of Immunology Research ( IF 4.1 ) Pub Date : 2021-08-04 , DOI: 10.1155/2021/7223500 Hai-Peng Wei 1 , Song Zhan 1 , Qing-An Zhu 1 , Zhen-Juan Chen 1 , Xian Feng 1 , Jun-Yuan Chen 2 , Qi-Lin Zhang 1, 3 , Jingjie Zhao 4 , Lingzhang Meng 5
Affiliation
Distinct expression of the miRNAs has rarely been explored in basal cell carcinoma (BCC) of skin, and the regulatory role of miRNAs in BCC development remains quite opaque. Here, we collected control tissues from adjacent noncancerous skin (; control group) and tissues at tumor centers from patients with cheek BCC (; BCC group) using punch biopsies. After six small RNA sequencing- (sRNA-seq-) based miRNA expression profiles were generated for both BCC and controls, including three biological replicates, we conducted comparative analysis on the sRNA-seq dataset, discovering 181 differentially expressed miRNAs (DEMs) out of the 1,873 miRNAs in BCCs. In order to validate the sRNA-seq data, expression of 15 randomly selected DEMs was measured using the TaqMan probe-based quantitative real-time PCR. Functional analysis of predicted target genes of DEMs in BCCs shows that these miRNAs are primarily involved in various types of cancers, immune response, epithelial growth, and morphogenesis, as well as energy production and metabolism, indicating that BCC development is caused, at least in part, by changes in miRNA regulation for biological and disease processes. In particular, the “basal cell carcinoma pathways” were found to be enriched by predicted DEM targets, and regulatory relationships between DEMs and their targeted genes in this pathway were further uncovered. These results revealed the association between BCCs and abundant miRNA molecules that regulate target genes, functional modules, and signaling pathways in carcinogenesis.
中文翻译:
基底细胞癌中微小RNA的全基因组表达差异
很少在皮肤基底细胞癌 (BCC) 中探索 miRNA 的不同表达,并且 miRNA 在 BCC 发展中的调节作用仍然非常不透明。在这里,我们从邻近的非癌性皮肤收集了对照组织(; 对照组)和脸颊 BCC 患者的肿瘤中心组织(;BCC 组)使用穿孔活检。在为 BCC 和对照生成六个基于小 RNA 测序 (sRNA-seq-) 的 miRNA 表达谱后,包括三个生物学重复,我们对 sRNA-seq 数据集进行了比较分析,发现了 181 个差异表达的 miRNA (DEM) BCC 中的 1,873 个 miRNA。为了验证 sRNA-seq 数据,使用基于 TaqMan 探针的定量实时 PCR 测量了 15 个随机选择的 DEM 的表达。对 BCC 中 DEM 的预测靶基因的功能分析表明,这些 miRNA 主要参与各种类型的癌症、免疫反应、上皮生长和形态发生,以及能量产生和代谢,表明 BCC 的发展是引起的,至少在部分,通过改变生物和疾病过程的 miRNA 调节。特别是,发现“基底细胞癌通路”被预测的 DEM 靶标富集,并进一步揭示了 DEM 与其靶基因在该通路中的调控关系。这些结果揭示了 BCC 和丰富的 miRNA 分子之间的关联,这些 miRNA 分子在致癌过程中调节靶基因、功能模块和信号通路。
更新日期:2021-08-04
中文翻译:
基底细胞癌中微小RNA的全基因组表达差异
很少在皮肤基底细胞癌 (BCC) 中探索 miRNA 的不同表达,并且 miRNA 在 BCC 发展中的调节作用仍然非常不透明。在这里,我们从邻近的非癌性皮肤收集了对照组织(; 对照组)和脸颊 BCC 患者的肿瘤中心组织(;BCC 组)使用穿孔活检。在为 BCC 和对照生成六个基于小 RNA 测序 (sRNA-seq-) 的 miRNA 表达谱后,包括三个生物学重复,我们对 sRNA-seq 数据集进行了比较分析,发现了 181 个差异表达的 miRNA (DEM) BCC 中的 1,873 个 miRNA。为了验证 sRNA-seq 数据,使用基于 TaqMan 探针的定量实时 PCR 测量了 15 个随机选择的 DEM 的表达。对 BCC 中 DEM 的预测靶基因的功能分析表明,这些 miRNA 主要参与各种类型的癌症、免疫反应、上皮生长和形态发生,以及能量产生和代谢,表明 BCC 的发展是引起的,至少在部分,通过改变生物和疾病过程的 miRNA 调节。特别是,发现“基底细胞癌通路”被预测的 DEM 靶标富集,并进一步揭示了 DEM 与其靶基因在该通路中的调控关系。这些结果揭示了 BCC 和丰富的 miRNA 分子之间的关联,这些 miRNA 分子在致癌过程中调节靶基因、功能模块和信号通路。