Ticks are important vector arthropods that transmit various pathogens to humans and other animals. Tick-borne viruses are of particular concern to public health as these are major agents of emerging and re-emerging infectious diseases. The Phenuiviridae family of tick-borne viruses is one of the most diverse groups and includes important human pathogenic viruses such as severe fever with thrombocytopenia syndrome virus. Phenuivirus-like sequences were detected during the surveillance of tick-borne viruses using RNA virome analysis from a pooled sample of Haemaphysalis formosensis ticks collected in Ehime, Japan. RT-PCR amplification and Sanger sequencing revealed the nearly complete viral genome sequence of all three segments. Comparisons of the viral amino acid sequences among phenuiviruses indicated that the detected virus shared 46%-70% sequence identity with known members of the Kaisodi group in the genus Uukuvirus. Furthermore, phylogenetic analysis of the viral proteins showed that the virus formed a cluster with the Kaisodi group viruses, suggesting that this was a novel virus, which was designated "Toyo virus" (TOYOV). Further investigation of TOYOV is needed, and it will contribute to understanding the natural history and the etiological importance of the Kaisodi group viruses.

中文翻译：

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东洋病毒是日本福尔摩斯蜱中发现的 Uukuvirus 属（Phenuiviridae 科）Kaisodi 组的新成员。

蜱是重要的媒介节肢动物，可将各种病原体传播给人类和其他动物。蜱传病毒是公众健康的特别关注点，因为它们是新出现和重新出现的传染病的主要病原体。蜱传病毒的 Phenuiviridae 家族是最多样化的群体之一，包括重要的人类致病病毒，如重症发热伴血小板减少综合征病毒。在监测蜱传病毒的过程中，使用 RNA 病毒组分析从日本爱媛收集的 Haemaphysalis formosensis 蜱的汇总样本中检测到了类病毒序列。RT-PCR 扩增和 Sanger 测序揭示了所有三个片段的几乎完整的病毒基因组序列。菲病毒中病毒氨基酸序列的比较表明，检测到的病毒与 Uukuvirus 属 Kaisodi 组的已知成员具有 46%-70% 的序列同一性。此外，病毒蛋白的系统发育分析表明该病毒与Kaisodi组病毒形成一个簇，表明这是一种新型病毒，被命名为“东洋病毒”（TOYOV）。需要对 TOYOV 进行进一步调查，这将有助于了解 Kaisodi 群病毒的自然历史和病因学重要性。