In solid-phase oligonucleotide synthesis, a single oligonucleotide is generally acquired from a column loaded with a specific solid support. Herein, we have developed new cleavable spacer (CS) derivatives for tandem synthesis of multiple oligonucleotides on a single column­. Four CS analogues were designed, synthesized, and inserted between two oligonucleotide sequences using an automated oligonucleotide synthesizer. The CS derivatives bearing a cyclic cis-1,2-diol exhibited efficient release of the two oligonucleotides under commonly employed basic conditions of aqueous ammonia. Among the CS analogues, it was found that CS with a robust structure can potentially be applied as a spacer molecule in the tandem synthesis of multiple oligonucleotides in a single sequence.

中文翻译：

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用于串联合成多种寡核苷酸的新型可切割间隔物

在固相寡核苷酸合成中，通常从装有特定固体支持物的柱子中获得单个寡核苷酸。在此，我们开发了新的可切割间隔 (CS) 衍生物，用于在单个柱上串联合成多个寡核苷酸。使用自动寡核苷酸合成仪设计、合成了四种 CS 类似物并插入到两个寡核苷酸序列之间。带有环状 cis-1,2-二醇的 CS 衍生物在常用的氨水碱性条件下表现出两种寡核苷酸的有效释放。在 CS 类似物中，发现具有稳健结构的 CS 可以潜在地用作间隔分子，用于单个序列中多个寡核苷酸的串联合成。