We introduce a ¹³C–²H Rotational Echo DOuble Resonance (REDOR) technique that uses the difference between on-resonance and off-resonance ²H irradiation to detect dynamic segments in deuterated molecules. By selectively inverting specific regions of the ²H magic-angle spinning (MAS) sideband manifold to recouple some of the deuterons to nearby carbons, we distinguish dynamic and rigid residues in 1D and 2D ¹³C spectra. We demonstrate this approach on deuterated GB1, H/D exchanged GB1, and perdeuterated bacterial cellulose. Numerical simulations reproduce the measured mixing-time and ²H carrier-frequency dependence of the REDOR dephasing of bacterial cellulose. Combining numerical simulations with experiments thus allow the extraction of motionally averaged quadrupolar couplings from REDOR dephasing values.

我们介绍了一种¹³ C – ² H 旋转回波双共振 (REDOR) 技术，该技术使用共振和非共振² H 辐射之间的差异来检测氘化分子中的动态片段。通过选择性地反转² H 魔角自旋 (MAS) 边带流形的特定区域以将一些氘核重新耦合到附近的碳，我们区分了一维和二维¹³ C 光谱中的动态和刚性残基。我们在氘化 GB1、H/D 交换 GB1 和过氘化细菌纤维素上演示了这种方法。数值模拟再现了测量的混合时间和²细菌纤维素的REDOR去相位的H 载波频率依赖性。因此，将数值模拟与实验相结合，可以从 REDOR 相移值中提取运动平均的四极耦合。