Silver nanoparticle synthesis of the leaf extract Tagetes erecta L. enriched with ascorbic acid and polyphenols has been investigated. The color of the golden yellow extract has changed to pinkish-brown due to the reduction of Ag⁺ to the colloidal solution of AgNPs and a sharp absorption peak at 420 nm under the UV-Vis spectrophotometer. In addition, the Fourier Transfer Infrared Spectroscopy (FTIR) estimation was completed in order to recognize and identify the biomolecules present in the extract acting as a reducing and capping agent for the AgNPs. The X-ray diffraction (XRD) peaks at (111), (201), (220), and (311) confirm the presence of monoclinic crystals in the solution. The morphology and size of the particles were provided by transmission electron microscopy (TEM) images of AgNPs. At a scale of 100 nm, synthesized AgNPs were predominantly spherical with a size range of 7-35 nm. In comparison to 7.39 mg/100 g in AgNPs, aqueous leaf extract was 55.14 mg/100 g higher in ascorbic acid. The phenolic and flavonoid content of extract was  mg (GAE/100 g) and  mg (QE/mL), and the colloidal AgNP solution was  mg (GAE/100 g) and  mg (QE/mL), respectively. Phenolic and flavonoid contents play a major role as a reducing agent and reduce the precursor AgNO₃ into AgNPs. The DPPH scavenging assay also assessed the antioxidant properties of extract and its derived AgNPs. As compared antioxidant value to aqueous leaf extract (mg/mL), higher percentage inhibition (PI) was found in AgNPs and free-radical scavenging activity of extract and AgNPs were directly linked to their concentrations. Results of this research have discovered a higher potential for free-radical scavenging AgNPs and will help to develop new and more potent antioxidants for the treatment of different diseases caused by oxidative stress; the higher antioxidant properties bearing AgNPs might be used.

中文翻译：

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抗坏血酸和多酚介导万寿菊水叶提取物银纳米颗粒的绿色合成并研究其抗氧化性能

叶提取物万寿菊的银纳米颗粒合成。已经研究了富含抗坏血酸和多酚的物质。由于Ag ⁺的减少，金黄色提取物的颜色变为粉棕色在 UV-Vis 分光光度计下，AgNPs 的胶体溶液和 420 nm 处的尖锐吸收峰。此外，还完成了傅里叶传输红外光谱 (FTIR) 估计，以识别和鉴定提取物中存在的生物分子，这些生物分子充当 AgNPs 的还原剂和封端剂。X 射线衍射 (XRD) 在 (111)、(201)、(220) 和 (311) 处的峰证实溶液中存在单斜晶体。颗粒的形态和尺寸由 AgNPs 的透射电子显微镜 (TEM) 图像提供。在 100 nm 的尺度下，合成的 AgNPs 主要是球形，尺寸范围为 7-35 nm。与 AgNPs 中的 7.39 mg/100 g 相比，水叶提取物的抗坏血酸含量高 55.14 mg/100 g。提取物中酚类和黄酮类物质的含量为 毫克（GAE/100 克）和  mg (QE/mL)，胶体 AgNP 溶液为  毫克（GAE/100 克）和  毫克 (QE/mL)，分别。酚类和类黄酮含量作为还原剂起主要作用，并将前体 AgNO ₃还原为 AgNPs。DPPH 清除试验还评估了提取物及其衍生的 AgNPs 的抗氧化特性。与水叶提取物 (mg/mL) 的抗氧化值相比，在 AgNPs 中发现了更高的抑制百分比 (PI)，并且提取物和 AgNPs 的自由基清除活性与其浓度直接相关。这项研究的结果发现了清除自由基的 AgNPs 具有更高的潜力，并将有助于开发新的、更有效的抗氧化剂，用于治疗由氧化应激引起的不同疾病；可以使用带有 AgNPs 的更高抗氧化性能。