Abstract—

Based on strains of various ecological and geographical origins, we studied intraspecific polymorphism of the wild yeast Kluyveromyces dobzhanskii, the closest relative of the cultured dairy yeasts K. lactis and K. marxianus. Using microsatellite typing, phylogenetic analysis of the nucleotide sequences of the ITS1/ITS2 internal transcribed spacers and of the mitochondrial gene COX2, we found the species K. dobzhanskii to be of complex composition, consisting of at least three geographical populations: North American (including the type culture CBS 2104), European, and Far Eastern. Strains of different geographic origin were characterized by unique nucleotide substitutions in the ITS1/ITS2 region and in the mitochondrial COX2 gene. In European strains, a correlation was revealed between the (GTG)₅ profiles and the source of isolation. The strains isolated from insects in Spain had unique patterns.

中文翻译：

---

酵母克鲁维酵母的分子遗传多态性

摘要——

基于各种生态和地理来源的菌株，我们研究了野生酵母Kluyveromyces dobzhanskii 的种内多态性，这是培养的乳酵母K. lactis和K. marxianus的最近亲。使用微卫星分型，对 ITS1/ITS2 内部转录间隔区和线粒体基因COX2的核苷酸序列进行系统发育分析，我们发现了物种K. dobzhanskii具有复杂的组成，至少由三个地理种群组成：北美（包括典型文化 CBS 2104）、欧洲和远东。不同地理来源的菌株的特征在于 ITS1/ITS2 区域和线粒体COX2基因中的独特核苷酸取代。在欧洲菌株中，揭示了 (GTG) ₅配置文件与隔离源之间的相关性。从西班牙昆虫中分离出的菌株具有独特的模式。