Abstract—

The possible nitrogen sources for Osc. trichoides DG6, a typical strain of the Oscillochloridaceae family, are ammonium, N₂, glutamate, asparagine, glycine, and glutamine. The assimilation of molecular nitrogen occurs with the participation of nitrogenase, the structural gene of which, nifH, is located in the gene cluster which also includes the genes of the nifD and nifK nitrogenase subunits and the auxiliary nifB gene. Considering that nifHBDK clusters have been also annotated in the genomes of other members of the Oscillochloridaceae family, including uncultured and candidate taxa, it can be assumed that the ability to fix nitrogen is a property immanent for this entire family. The pathways for assimilating ammonium in the cells grown using different nitrogen sources may differ. Osc. trichoides DG6 growing in a medium containing ammonium assimilated it with the participation of glutamate dehydrogenase, which is determined by a single gene. The expression product of this gene has dual functionality and can be used to implement the reaction with both NAD and NADP. With the growth of Osc. trichoides DG6 on a medium with glutamate as the only nitrogen source all the enzymes necessary for the implementation of the GS‑GOGAT pathway were found in the cells. However, for the glutamine synthetase reaction, ammonium, which was absent in the growth medium, was required. The source of ammonium may be glutamate metabolized through glutamate dehydrogenase.

中文翻译：

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无氧丝状光养细菌 Oscillocholris trichoides DG-6 菌株的氮代谢

摘要——

Osc可能的氮源。trichoides DG6 是Oscillochloridaceae科的典型菌株，含有铵、N ₂、谷氨酸盐、天冬酰胺、甘氨酸和谷氨酰胺。分子氮的同化是在固氮酶的参与下发生的，其结构基因nif H 位于基因簇中，该基因簇还包括nif D 和nif K 固氮酶亚基的基因和辅助nif B 基因。考虑到nif HBDK 簇也已在Oscillochloridaceae的其他成员的基因组中进行了注释家庭，包括未培养的和候选分类群，可以假设固氮能力是整个家庭固有的财产。在使用不同氮源生长的细胞中吸收铵的途径可能不同。振荡器 在含有铵的培养基中生长的trichoides DG6 在谷氨酸脱氢酶的参与下同化它，谷氨酸脱氢酶由单个基因决定。该基因的表达产物具有双重功能，可用于实现与NAD和NADP的反应。随着Osc的成长。三线体DG6 在以谷氨酸盐作为唯一氮源的培养基上，在细胞中发现了实施 GS-GOGAT 途径所需的所有酶。然而，对于谷氨酰胺合成酶反应，需要生长培养基中不存在的铵。铵的来源可以是通过谷氨酸脱氢酶代谢的谷氨酸。