Immobilization of microbe cells has been considered as a potential alternative for enhancing biobutanol productivity through acetone-butanol-ethanol (ABE) fermentation. Vetiver grass (VG; Vetiveria zizanoides) pretreated by sodium hydroxide (NaOH) or sulphuric acid was used as carrier for immobilized Clostridium beijerinckii TISTR 1461 in ABE fermentation. The removal of the lignin and hemicellulose fractions of VG during the pretreatment resulted in structural changes, especially an increased crystallinity and surface area. The VG pretreated with 6% (w/v) NaOH at 120 °C for 6 h had the best potential as a carrier in ABE fermentation, giving a butanol and total ABE concentration of 11.6 g/L and 19.8 g/L, respectively, approximately 1.3-fold greater than that of the free-cell system. The VG samples were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, and surface area analyses.

微生物细胞的固定化已被认为是通过丙酮-丁醇-乙醇 (ABE) 发酵提高生物丁醇生产力的潜在替代方案。用氢氧化钠（NaOH）或硫酸预处理的香根草（VG；Vetiveria zizanoides）作为固定化拜氏梭菌的载体ABE 发酵中的 TISTR 1461。在预处理过程中去除 VG 的木质素和半纤维素部分会导致结构变化，尤其是结晶度和表面积增加。用 6% (w/v) NaOH 在 120 °C 预处理 6 h 的 VG 作为 ABE 发酵中的载体具有最佳潜力，产生的丁醇和总 ABE 浓度分别为 11.6 g/L 和 19.8 g/L，大约是自由细胞系统的 1.3 倍。VG 样品通过 X 射线衍射、傅里叶变换红外光谱、热重分析、扫描电子显微镜和表面积分析进行表征。