Background Cleaning is very important in reusable surgical instruments (RSI) reprocessing. The adenosine triphosphate (ATP) method is widely used to assess cleanliness, but few studies focus on using this method on RSIs. Objective To assess the effectiveness of the ATP Bioluminescence Assay for monitoring the cleanliness of RSIs. Methods The study was conducted in three central sterile supply departments (CSSD) from 2015 to 2020. Surgical scissors, hemostatic forceps, and other non-lumen instruments were sampled after cleaning. The surface of each instrument was swabbed in a uniform manner, including hinged joints and teeth, and the amount of ATP (amol) was calculated. Results A total of 871 RSIs were tested, the mean ATP lg (amol) was 2.829 ± 0.539 and the qualified rate was 80.57%. Washer-disinfector cleaning was more efficient than manual cleaning, the mean ATP lg (amol) were 2.776 ± 0.513 and 2.948 ± 0.575, respectively; the qualified rates were 84.97% and 70.59%, respectively. With the time of bare instruments expose to environment increased, the qualified rate was decreased (Ptrend = 0.044). Conclusion ATP levels are stable and repeatable for continuous monitoring of the cleanliness of RSIs. It is a rapid and viable method for assessing the cleanliness of RSIs. Washer-disinfector cleaning is recommended and cleaned instruments should be placed in sterile packaging in good time. Highlights The ATP method is viable for assessing the cleanliness of RSI in CSSDs.

中文翻译：

---

三磷酸腺苷法监测中央无菌供应部可重复使用手术器械的清洁度

背景清洁在可重复使用的手术器械 (RSI) 再处理中非常重要。三磷酸腺苷 (ATP) 方法广泛用于评估清洁度，但很少有研究关注在 RSI 上使用这种方法。目的评估 ATP 生物发光检测法用于监测 RSI 清洁度的有效性。方法 2015-2020年在三个中央无菌供应部门（CSSD）开展研究，对手术剪、止血钳等非管腔器械进行清洗后取样。以均匀的方式擦拭每个仪器的表面，包括铰接关节和牙齿，并计算ATP（amol）的量。结果共检测871个RSI，平均ATP lg（amol）为2.829±0.539，合格率为80.57%。清洗消毒器清洗比人工清洗更有效，平均 ATP lg (amol) 分别为 2.776 ± 0.513 和 2.948 ± 0.575；合格率分别为84.97%和70.59%。随着裸器械暴露在环境中的时间增加，合格率下降（Ptrend = 0.044）。结论 ATP 水平稳定且可重复，可用于连续监测 RSI 的清洁度。这是评估 RSI 清洁度的一种快速可行的方法。建议使用清洗消毒器进行清洗，清洗过的器械应及时放入无菌包装中。要点 ATP 方法可用于评估 CSSD 中 RSI 的清洁度。随着裸器械暴露在环境中的时间增加，合格率下降（Ptrend = 0.044）。结论 ATP 水平稳定且可重复，可用于连续监测 RSI 的清洁度。这是评估 RSI 清洁度的一种快速可行的方法。建议使用清洗消毒器进行清洗，清洗过的器械应及时放入无菌包装中。要点 ATP 方法可用于评估 CSSD 中 RSI 的清洁度。随着裸器械暴露在环境中的时间增加，合格率下降（Ptrend = 0.044）。结论 ATP 水平稳定且可重复，可用于连续监测 RSI 的清洁度。这是评估 RSI 清洁度的一种快速可行的方法。建议使用清洗消毒器进行清洗，清洗过的器械应及时放入无菌包装中。要点 ATP 方法可用于评估 CSSD 中 RSI 的清洁度。建议使用清洗消毒器进行清洗，清洗过的器械应及时放入无菌包装中。要点 ATP 方法可用于评估 CSSD 中 RSI 的清洁度。建议使用清洗消毒器进行清洗，清洗过的器械应及时放入无菌包装中。要点 ATP 方法可用于评估 CSSD 中 RSI 的清洁度。