ABSTRACT

The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is an agricultural important polyphagous pest of many crops that causes significant yield loss. In this study, the effect of four herbivore-induced plant volatiles (HIPVs) (methyl jasmonate, methyl salicylate, indole, and 3-pentanol) with a concentration of 100 mmol/L and two plant probiotic bacteria (PBB) (Bacillus pumilus INR7 and Bacillus velezensis H3), were investigated on life table parameters of T. urticae under laboratory conditions (25 ± 1°C, 65 ± 5% RH, and 16 L: 8D). The T. urticae cohorts were reared on attached leaves of kidney bean plants (Phaseolus vulgaris L.) treated with HIPVs and PBBs, separately. The life history data were analysed using age-stage, two-sex life table theory. The intrinsic rate of increase (r) on methyl salicylate (0.1667 ± 0.0087 day-1) and B. velezensis treatment (0.1640 ± 0.0099 day-1) was significantly lower than the control group (0.1934 ± 0.0100 day-1). The finite rate of increase (λ) ranged from 1.178 ± 0.0116 day-1 on the B. velezensis to 1.2134 ± 0.0121 day-1 on the control with a significant difference (P < 0.05). The gross reproduction rate (GRR) on the indole treatment was significantly lower than the other treatments (28.14 ± 3.66 offspring). Based on the results, methyl salicylate, B. velezensis and indole could be considered resistance inducing materials against T. urticae.

摘要

二斑叶螨Tetranychus urticae Koch (Acari: Tetranychidae) 是许多作物的农业重要杂食性害虫，会导致显着的产量损失。在本研究中，浓度为 100 mmol/L 的四种草食动物诱导的植物挥发物 (HIPV)（茉莉酸甲酯、水杨酸甲酯、吲哚和 3-戊醇）和两种植物益生菌（PBB）（短小芽孢杆菌INR7和芽孢杆菌velezensis H3），考察了的生命表参数T.螨实验室条件下（25±1℃，65±5％RH，和16 L：8D）。该T.螨同伙被饲养在菜豆植物的叶相连（菜豆L.) 分别用 HIPV 和 PBB 处理。使用年龄阶段、两性生命表理论分析生活史数据。水杨酸甲酯 (0.1667 ± 0.0087 day-1) 和B. velezensis治疗 (0.1640 ± 0.0099 day-1)的内在增长率 ( r )显着低于对照组 (0.1934 ± 0.0100 day-1)。有限的增长率 (λ) 范围从B. velezensis 的1.178 ± 0.0116 day-1到对照的 1.2134 ± 0.0121 day-1，差异显着（P < 0.05）。吲哚处理的总繁殖率 ( GRR ) 显着低于其他处理（28.14 ± 3.66 个后代）。根据结果​​，水杨酸甲酯、B. velezensis和吲哚可以被认为是针对抗性诱导物质T.螨。