The aim of this study was to compare the elimination of Bordetella pertussis clinical isolates, representing different genotypes in relation to alleles encoding virulence factors (MLST—multi-locus antigen sequence typing), MLVA type (multi-locus variable-number tandem repeat analysis) and PFGE group (pulsed-field gel electrophoresis) from the lungs of naive mice or mice were immunised with the commercial whole-cell pertussis vaccine, the acellular pertussis vaccine and the experimental whole-cell pertussis vaccine. Molecular data indicate that the resurgence of pertussis in populations with high vaccine coverage is associated with genomic adaptation of B. pertussis, to vaccine selection pressure. Pertactin-negative B. pertussis isolates were suspected to contribute to the reduced vaccine effectiveness. It was shown that one of the isolates used is PRN deficient. The mice were intranasally challenged with bacterial suspension containing approximately 5 × 10 ⁷ CFU/ml B. pertussis. The immunogenicity of the tested vaccines against PT (pertussis toxin), PRN (pertactin), FHA (filamentous haemagglutinin) and FIM (fimbriae types 2 and 3) was examined. The commercial whole-cell and acellular pertussis vaccines induced an immunity effective at eliminating the genetically different B. pertussis isolates from the lungs. However, the elimination of the PRN-deficient isolate from the lungs of mice vaccinated with commercial vaccines was delayed as compared to the PRN ( +) isolate, suggesting phenotypic differences with the circulating isolates and vaccine strains. The most effective vaccine was the experimental vaccine with the composition identical to that of the strains used for infection.

本研究的目的是比较百日咳博德特氏菌 临床分离株的消除情况，代表与编码毒力因子的等位基因（MLST-多位点抗原序列分型）、MLVA 型（多位点可变数量串联重复分析）相关的不同基因型用市售全细胞百日咳疫苗、无细胞百日咳疫苗和实验性全细胞百日咳疫苗免疫来自幼稚小鼠或小鼠肺部的PFGE组和PFGE组（脉冲场凝胶电泳）。分子数据表明，疫苗覆盖率高的人群中百日咳的死灰复燃与百日咳杆菌对疫苗选择压力的基因组适应有关。Pertactin 阴性百日咳杆菌怀疑分离株导致疫苗有效性降低。结果表明，使用的分离株之一是缺乏 PRN。^{用含有大约 5×10 7}  CFU/ml百日咳杆菌的细菌悬浮液对小鼠进行鼻内攻击。检测了针对 PT（百日咳毒素）、PRN（pertactin）、FHA（丝状血凝素）和 FIM（2 型和 3 型菌毛）的测试疫苗的免疫原性。商业化的全细胞和无细胞百日咳疫苗可诱导免疫，有效消除基因不同的百日咳杆菌从肺中分离出来。然而，与 PRN (+) 分离物相比，从接种商业疫苗的小鼠肺中消除 PRN 缺陷分离物的时间有所延迟，这表明与循环分离物和疫苗株存在表型差异。最有效的疫苗是与用于感染的菌株组成相同的实验疫苗。