Polysaccharides (SLP) derived from Sparassis latifolia were depolymerised by ultrasonication, hydrochloric acid, and H₂O₂/Vc to obtain the depolymerised polysaccharides U-SLP, H-SLP, and HV-SLP, respectively. Results from X-ray powder diffraction, atomic force microscopy, Fourier-transfrom infrared spectroscopy, proton nuclear magnetic resonance spectroscopy and scanning electron microscopy indicated that the SLP were damaged to varying degrees by the three methods. The results showed that the three depolymerisation methods broke down the molecular chains, impeded aggregation, and reduced the molecular weight of the polysaccharides without changing their primary structure and monosaccharide composition of SLP. Relative to the original SLP, the depolymerised SLPs showed higher antioxidant activity during in vitro ABTS and reducing power assays. Also, the depolymerised SLPs could effectively improve the survival rate of zebrafish and reduce their heart rate, reactive oxygen species production, and cell death. The HV-SLP had the highest antioxidant activity of the three depolymerised fractions. The results revealed that the H₂O₂/Vc treatment is an effective method for preparing depolymerised SLP products with low molecular weights and dramatic antioxidant activity.

通过超声波、盐酸和 H ₂ O ₂解聚来自阔叶松的多糖 (SLP)/Vc 分别获得解聚多糖 U-SLP、H-SLP 和 HV-SLP。X射线粉末衍射、原子力显微镜、傅里叶变换红外光谱、质子核磁共振光谱和扫描电子显微镜的结果表明，三种方法对SLP都有不同程度的破坏。结果表明，三种解聚方法在不改变多糖的一级结构和单糖组成的情况下，破坏了分子链，阻碍了多糖的聚集，降低了多糖的分子量。相对于原始 SLP，解聚的 SLP在体外表现出更高的抗氧化活性ABTS 和还原能力测定。此外，解聚的 SLP 可以有效提高斑马鱼的存活率，降低其心率、活性氧的产生和细胞死亡。HV-SLP 在三个解聚部分中具有最高的抗氧化活性。结果表明，H ₂ O ₂ /Vc 处理是制备具有低分子量和显着抗氧化活性的解聚SLP 产品的有效方法。