In many sensitive assays, target molecules are tagged using fluorescently labeled probes and captured using magnetic beads. Here, we introduce an optical modulation biosensing (OMB) system, which aggregates the beads into a small detection area and separates the signal from the background noise by manipulating the laser beam in and out of the cluster of beads. Using the OMB system to detect human interleukin-8, we demonstrated a limit of detection of 0.02 ng/L and a 4-log dynamic range. Using Zika-positive and healthy individuals’ serum samples, we show that the OMB-based Zika IgG serological assay has 96% sensitivity and 100% specificity.

中文翻译：

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用于快速检测低浓度生物靶标的光学调制生物传感系统

在许多敏感分析中，目标分子使用荧光标记的探针进行标记，并使用磁珠进行捕获。在这里，我们引入了一种光学调制生物传感 (OMB) 系统，该系统将珠子聚集到一个小的检测区域中，并通过操纵进出珠子簇的激光束将信号与背景噪声分开。使用 OMB 系统检测人类白细胞介素 8，我们展示了 0.02 ng/L 的检测限和 4-log 动态范围。使用寨卡病毒阳性和健康个体的血清样本，我们表明基于 OMB 的寨卡 IgG 血清学检测具有 96% 的灵敏度和 100% 的特异性。