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Assessment of biologically active compounds, organic acids and antioxidant activity in needle extracts of different Norway spruce (Picea abies (L.) H. Karst) half-sib families
Wood Science and Technology ( IF 3.4 ) Pub Date : 2021-07-31 , DOI: 10.1007/s00226-021-01322-5
Ieva Lučinskaitė 1 , Jonas Žiauka 1 , Virgilijus Baliuckas 1 , Vytautas Čėsna 1 , Vaida Sirgedaitė-Šėžienė 1 , Kristina Laužikė 2
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The differences of total phenolic content (TPC), total flavonoid content (TFC), photosynthetic pigment, organic acids and antioxidant activity in Norway spruce (Picea abies (L.) H. Karst) needle extracts were determined to compare different half-sib spruce families’ chemical backgrounds and the abilities to synthesize biologically active compounds and antioxidant activity for potential pharmaceutical and nutraceutical use. Spectrophotometric analysis showed that the highest TPC was found in needle extracts of half-sib family 599 (38.7 mg g−1). The needle extracts of family 548 had the highest TFC (797.7 mg g−1). The highest concentration of chlorophyll a (chl a), chlorophyll b (chl b) and carotenoids was determined in the needle extracts of Norway spruce families 541 (0.651 mg g−1, 0.386 mg g−1 and 0.235 mg g−1, respectively) and 463 (0.655 mg g−1, 0.383 mg g−1 and 0.227 mg g−1, respectively). Using HPLC analysis, the highest concentrations of vitamin C and folic acid were determined in needle extracts of family 541 (16.93 mg g−1 and 0.02 mg g−1, respectively), the highest concentrations of malic and succinic acids were found in 463 (0.95 mg g−1 and 3.09 mg g−1, respectively), the highest concentration of oxalic acid was identified in needle extracts of family 599 (0.50 mg g−1), and the highest concentration of citric acid was found in needle extracts of family 577 (0.68 mg g−1).



中文翻译:

评估不同挪威云杉 (Picea abies (L.) H. Karst) 半同胞家族的针叶提取物中的生物活性化合物、有机酸和抗氧化活性

测定挪威云杉(Picea abies (L.) H. Karst)针叶提取物中总酚含量(TPC)、总黄酮含量(TFC)、光合色素、有机酸和抗氧化活性的差异,以比较不同半同胞云杉家庭的化学背景以及合成生物活性化合物和抗氧化活性的能力,以用于潜在的药物和营养品用途。分光光度法分析表明,在半同胞家族 599 的针提取物中发现了最高的 TPC(38.7 mg g -1)。家族 548 的针提取物具有最高的 TFC (797.7 mg g -1 )。叶绿素a (chl a )、叶绿素b (chlb ) 和类胡萝卜素在挪威云杉科 541(分别为 0.651 mg g -1、0.386 mg g -1和 0.235 mg g -1)和 463(0.655 mg g -1、0.383 mg g -1)的针叶提取物中测定和 0.227 mg g -1)。使用 HPLC 分析,在 541 科的针叶提取物中测定了最高浓度的维生素 C 和叶酸(分别为 16.93 mg g -1和 0.02 mg g -1),在 463 科中发现了最高浓度的苹果酸和琥珀酸( 0.95 毫克 g -1和 3.09 毫克 g -1分别),在家族 599 的针叶提取物中发现最高浓度的草酸(0.50 mg g -1),在家族 577 的针叶提取物中发现最高浓度的柠檬酸(0.68 mg g -1)。

更新日期:2021-08-01
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