Background

Cortex Phellodendri amurensis (CPA) has high medicinal value in the treatment of kidney-yin deficiency diseases. However, due to the lack of research on the therapeutic material basis of CPA, the current quality control standard for CPA is defective, and the effect of the nourishing kidney-yin of CPA was limited.

Purpose

Based on the principle of correspondence between the syndrome and prescriptions, we studied the CPA in ZhibaiDihuang pill (ZBDH) to identify quality markers (Q-markers) of CPA in ZBDH for treating kidney-yin deficiency and seek the potential Q-markers of CPA under nourishing kidney-yin effect combined with the analysis of single CPA.

Methods

Taking Chinmedomics as the core strategy, metabonomics analysis and effective component identification were performed by UPLC-MS.

Results

A total of 121 chemical components of ZBDH were identified, among which the contents of berberine, palmatine, jatrorrhizine and magnoflorine changed the most obviously with the addition of CPA. Forty-five components were identified in the blood in the markedly effective state, including berberine, palmatine, jatrorrhizine and magnoflorine. The therapeutic material basis of ZBDH in the treatment of kidney-yin deficiency was found, and 6 components were found to derive from CPA, including magnoflorine and jatrorrhizine. In addition, seventeen components were identified in the blood in the single CPA treatment, including berberine, palmatine, jatrorrhizine and magnoflorine.

Conclusions

Magnoflorine and jatrorrhizine were the Q-markers of CPA for treating kidney-yin deficiency in the formula of ZBDH and they were also potential Q-markers of the nourishing kidney-yin of CPA.

中文翻译：

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基于中药组学策略破译知白地黄丸滋补肾阴的Q标志物

背景

黄柏（CPA）在治疗肾阴虚病方面具有很高的药用价值。但由于缺乏对CPA治疗物质基础的研究，现行CPA质量控制标准存在缺陷，CPA滋补肾阴的作用有限。

目的

本着证候对应的原则，我们对知白地黄丸（ZBDH）中的CPA进行了研究，以确定ZBDH中CPA治疗肾阴虚的质量标志物（Q-markers），寻找CPA的潜在Q-markers在滋补肾阴作用下结合单次CPA分析。

方法

以中药组学为核心策略，采用UPLC-MS进行代谢组学分析和有效成分鉴定。

结果

共鉴定出ZBDH的121种化学成分，其中小檗碱、巴马汀、药根碱和木兰素的含量随CPA的加入变化最为明显。在显着有效状态的血液中鉴定出 45 种成分，包括小檗碱、巴马汀、药根碱和木兰素。发现了ZBDH治疗肾阴虚的治疗物质基础，发现6种成分来源于CPA，包括木兰花素和麻根碱。此外，在单次 CPA 治疗中，在血液中鉴定出 17 种成分，包括小檗碱、巴马汀、药根碱和木兰素。

结论

厚朴素和药根碱是ZBDH方中CPA治疗肾阴虚的Q标志物，也是CPA滋补肾阴的潜在Q标志物。