Hyphantria cunea (Drury) (Lepidoptera: Noctuidae) is a main pest of forest trees. In this study, the effects of Serratia marcescens Bizio (SM1) infection on the transcriptome of H. cunea were studied. The expression of 1068 unigenes in the transcriptome of H. cunea infected by S. marcescens was markedly different from that in the control of H. cunea; 474 genes were upregulated, and 594 genes were downregulated in the former. Among them, 8 cytochrome P450s (CYPs), 5 uridine diphosphate-glycosyltransferases (UGTs) and 3 glutathione S-transferases (GSTs) were significantly differentially expressed. Pathway enrichment analysis indicated that these differentially expressed detoxification enzyme genes were mainly involved in the drug metabolism pathway, glutathione metabolism pathway and ABC transporter pathway. Interestingly, we found that five UGTs were related to oestradiol metabolism in the steroid hormone biosynthesis pathway. Furthermore, the real-time fluorescent quantitative PCR results showed that SM1 could induce the expression of CYPs and UGTs, but inhibit the expression of GSTs. This research will identify the response of important detoxification enzymes to S. marcescens, which will provide a theoretical foundation for the development of new immunosuppressants for H. cunea control. Furthermore, H. cunea was performed transcriptome sequencing to explore the key metabolic pathways, signalling pathways and genes affected by S. marcescens, which will clarify the mechanisms of S. marcescens infection of H. cunea. In addition, this study also explored the relationship between H. cunea and S. marcescens, which will provide a theoretical basis for the biological control of H. cunea by using S. marcescens.

Hyphantria cunea（Drury）（鳞翅目：夜蛾科）是林木的主要害虫。在这项研究中，研究了粘质沙雷氏菌(SM1) 感染对H. cunea转录组的影响。S. marcescens感染的H. cunea转录组中 1068 个 unigenes 的表达与对照H. cunea有显着差异; 474个基因上调，594个基因下调。其中，8种细胞色素P450s（CYPs）、5种尿苷二磷酸糖基转移酶（UGTs）和3种谷胱甘肽S-转移酶（GSTs）显着差异表达。通路富集分析表明，这些差异表达的解毒酶基因主要参与药物代谢通路、谷胱甘肽代谢通路和ABC转运蛋白通路。有趣的是，我们发现五个 UGT 与类固醇激素生物合成途径中的雌二醇代谢有关。此外，实时荧光定量PCR结果显示SM1可诱导CYPs和UGTs的表达，但抑制GSTs的表达。这项研究将确定重要的解毒酶对S. marcescens，这将为开发用于H. cunea控制的新型免疫抑制剂提供理论基础。此外，H.美国白蛾进行转录组测序来探索关键代谢途径，信号受途径和基因粘质沙雷氏菌，这将澄清的机制粘质沙雷氏菌感染的H.美国白蛾。此外，本研究还探讨了楔形棘球蚧与粘质棘球蚧之间的关系，为利用粘质棘球蚴生物防治楔形棘球蚴提供理论依据。