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A ball microscope for viewing the entire surface of amphibian embryos
Biosystems ( IF 1.6 ) Pub Date : 2021-07-31 , DOI: 10.1016/j.biosystems.2021.104498
Susan J Crawford-Young 1 , Janet L Young Williment 2
Affiliation  

Waves on the surface of developing eggs/embryos need to be viewed from all sides of their 3D tissue. The ball microscope will enable tracking of cellular waves and determine their interactions with the cells on the surface. Nine microscopes are arrayed in a spherical formation around an imaging stage to create whole surface images of objects anywhere from 0.5 mm3 to 60 mm3 in size. The 3D printed ball-based microscope is made using nine, Opti-Tekscope OT-HD Digital USB Microscope Camera Magnifiers. Eight of the microscope cameras fit into the ball at 90° angles to each other and one bottom microscope is used for a base to hold the stage. The base will support a customised cuvette to hold the embryo in water. The microscopes are the size of a pen (13 cm long and 1 cm in diameter) and each have a ring light around their diameter for self illumination. The nine microscopes can be attached to a microcontroller for time-lapse automated imaging. This microscope will be compared to other microscopes developed for the same purpose. The microscope can be used for time lapse imaging of the surface of small 3D objects and can be used to view Axolotl salamander embryo development as the Axolotl embryos are 2 mm in diameter. Other amphibian eggs can also be imaged using this technique.



中文翻译:

用于观察两栖动物胚胎整个表面的球显微镜

需要从其 3D 组织的所有侧面观察发育中的卵子/胚胎表面上的波。球显微镜将能够跟踪细胞波并确定它们与表面细胞的相互作用。九台显微镜以球形形式排列在成像台周围,以创建从 0.5 mm 3到 60 mm 3的任何物体的整个表面图像在尺寸方面。3D 打印的基于球的显微镜由九个 Opti-Tekscope OT-HD 数字 USB 显微镜相机放大镜制成。八台显微镜摄像机以 90° 角安装在球中,一台底部显微镜用于固定载物台的底座。底座将支持一个定制的比色皿,以将胚胎保持在水中。这些显微镜只有一支笔那么大(长 13 厘米,直径 1 厘米),每个显微镜的直径周围都有一个环形灯,用于自发光。九个显微镜可以连接到微控制器以进行延时自动成像。该显微镜将与为相同目的开发的其他显微镜进行比较。该显微镜可用于小型 3D 物体表面的延时成像,并可用于观察蝾螈胚胎发育,因为蝾螈胚胎的直径为 2 毫米。

更新日期:2021-08-03
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