Parrotiopsis jacquemontiana (Decne) Rehder aqueous extract of leaf was used for biosynthesis of AgNPs and characterized through UV–Visible spectroscopy, X-ray diffraction, Fourier-transform-infrared, diffraction light scattering, and scanning electron microscope analysis. Moderate to strong antioxidant activity during in vitro antioxidant assays for scavenging of 2,2-diphenyl-1-picryl hydrazyl (DPPH), hydroxyl (OH), nitric oxide (NO) radicals, iron chelation, and inhibition of β-carotene bleaching was recorded with minimum IC₅₀ value (27.70 ± 2.67 μg/ml) calculated for OH radicals. The AgNPs were evaluated against six multidrug resistant human bacterial strains and minimum inhibitory concentration (MIC) along with minimum bactericidal concentration (MBC) values was determined and all were found remarkably susceptible. The bacterial strain Staphylococcus aureus was the most susceptible with MIC = 5 μg/ml and MBC = 10 μg/ml. Among six fungal strains, Fumigatus esculentum was the most susceptible with MIC and MBC of 10 μg/ml. 3-(4,5-Dimethylthiazol-2 yl)-2,5-diphenyltetrazolium bromide (MTT) screening assay against cancer cell lines (HCCLM3, HEPG2, MDA-MB 231, and MCF-7) revealed the least IC₅₀ values against HCCLM3 (74.20 ± 5.04) and MCF-7 (91.90 ± 1.17). While no cytotoxicity against normal cell lines; LO2 and MCF-10a was recorded. Parrotiopsis jacquemontiana silver nanoparticles (PJAgNPs) significantly (p > .001) prevented the migration of HCCLM3 cells in a dose-dependent style, relative to control. The wound healing potential of AgNPs in rat was found higher (p < .05) for wound contraction rates, hydroxyproline content, hemostatic and re-epithelization and regeneration efficiency in comparison to the reference group.

中文翻译：

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来自 Parrotiopsis jacquemontiana (Decne) Rehder 叶提取物的绿色合成银纳米粒子 (AgNPs) 及其生物活性

Parrotiopsis jacquemontiana (Decne) Rehder 叶的水提取物用于 AgNPs 的生物合成，并通过紫外-可见光谱、X 射线衍射、傅里叶变换红外、衍射光散射和扫描电子显微镜分析进行表征。在用于清除 2,2-二苯基-1-苦基肼 (DPPH)、羟基 (OH)、一氧化氮 (NO) 自由基、铁螯合和抑制 β-胡萝卜素漂白的体外抗氧化试验中具有中等至强的抗氧化活性。以最低 IC ₅₀记录OH 自由基的计算值 (27.70 ± 2.67 μg/ml)。AgNPs 针对六种多重耐药的人类细菌菌株进行了评估，并确定了最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC) 值，发现所有这些都非常敏感。金黄色葡萄球菌菌株最敏感，MIC = 5 μg/ml 和 MBC = 10 μg/ml。在六个真菌菌株中，Fumigatus esculentum是最敏感的，MIC 和 MBC 为 10 μg/ml。针对癌细胞系（HCCLM3、HEPG2、MDA-MB 231 和 MCF-7）的 3-(4,5-二甲基噻唑-2 基)-2,5-二苯基溴化四唑 (MTT) 筛选试验显示 IC ₅₀最低HCCLM3 (74.20 ± 5.04) 和 MCF-7 (91.90 ± 1.17) 的值。虽然对正常细胞系没有细胞毒性；记录了 LO2 和 MCF-10a。Parrotiopsis jacquemontiana银纳米粒子 (PJAgNPs) 显着 ( p  > .001) 相对于对照以剂量依赖性方式阻止了 HCCLM3 细胞的迁移。 与参照组相比，在伤口收缩率、羟脯氨酸含量、止血和再上皮化和再生效率方面，AgNPs 在大鼠中的伤口愈合潜力更高 ( p < .05)。