BACKGROUND Many hormones display distinct circadian rhythms, driven by central regulators, hormonal bioavailability, and half-life. A set of 11-oxygenated C19 steroids (11-oxyandrogens) and pregnenolone sulfate (PregS) are elevated in congenital adrenal hyperplasia and other disorders, but their circadian patterns have not been characterized. PARTICIPANTS AND METHODS Peripheral blood was collected every 2 h over 24 h from healthy volunteer men (10 young, 18-30 years, and 10 older, 60-80 years). We used mass spectrometry to quantify 15 steroids, including androstenedione (A4), testosterone (T), 11β-hydroxy- and 11-ketotestosterone (11OHT, 11KT),11β-hydroxy- and 11-ketoandrostenedione (11OHA4, 11KA4), and 4 ∆5-steroid sulfates. Diurnal models including mesor (rhythm adjusted median), peak, and nadir concentrations, acrophase, and amplitude were computed. RESULTS 11OHA4 followed a rhythm similar to cortisol: acrophase 8:00 h, nadir 21:00 h and were similar in young and old men. 11KT had similar diurnal patterns, but the peak was lower in older than in young men, as was the case for A4. All four steroid sulfates were higher in young vs older men. PregS and 17-hydroxypregnenolone sulfate (17OHPregS) showed sustained elevations between 8:00 and 18:00 h, and nadirs around midnight, while DHEAS and AdiolS displayed minimal diurnal variations. All 4 11-oxyandrogens correlated tightly with cortisol (r from 0.54 for 11OHT to 0.81 for 11OHA4, P < 0.0001 for all), but very weakly with T, supporting their adrenal origin and ACTH governance. CONCLUSIONS 11-Oxyandrogens, PregS, and 17OHPregS display distinct circadian and age variations, which should be accounted for when used as clinical biomarkers.

中文翻译：

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健康男性中 11-氧合 C19 类固醇和 Δ5-类固醇硫酸盐的昼夜节律。

背景许多激素表现出不同的昼夜节律，由中枢调节剂、激素生物利用度和半衰期驱动。一组 11-氧合 C19 类固醇（11-氧雄激素）和孕烯醇酮硫酸盐 (PregS) 在先天性肾上腺增生和其他疾病中升高，但它们的昼夜节律模式尚未确定。参与者和方法 在 24 小时内每 2 小时从健康的男性志愿者（10 名年轻，18-30 岁，10 名年长，60-80 岁）收集外周血。我们使用质谱法对 15 种类固醇进行量化，包括雄烯二酮 (A4)、睾酮 (T)、11β-羟基和 11-酮睾酮 (11OHT、11KT)、11β-羟基和 11-酮雄烯二酮 (11OHA4、11KA4) 和 4 Δ5-类固醇硫酸盐。昼夜模型，包括中值（节律调整中值）、峰值和最低浓度、顶相、并计算幅度。结果 11OHA4 遵循类似于皮质醇的节律：顶峰 8:00 小时，最低点 21:00 小时，并且在年轻和老年男性中相似。11KT 具有相似的昼夜模式，但老年人的峰值低于年轻男性，A4 也是如此。年轻男性与年长男性的所有四种类固醇硫酸盐均较高。PregS 和 17-羟基孕烯醇酮硫酸盐 (17OHPregS) 在 8:00 和 18:00 之间表现出持续升高，在午夜左右达到最低点，而 DHEAS 和 AdiolS 表现出最小的昼夜变化。所有 4 种 11-氧雄激素与皮质醇密切相关（r 从 11OHT 的 0.54 到 11OHA4 的 0.81，所有 P < 0.0001），但与 T 非常弱相关，支持它们的肾上腺起源和 ACTH 治理。结论 11-Oxyandrogens、PregS 和 17OHPregS 表现出明显的昼夜节律和年龄变化，