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VvBRC inhibits shoot branching in grapevine
Scientia Horticulturae ( IF 4.3 ) Pub Date : 2021-07-30 , DOI: 10.1016/j.scienta.2021.110370
Zhuo Min 1 , Li Chen 1 , Yang Zhang 2 , Ziyu Li 1 , Min Liu 1 , Wan Ping Li 1 , Yanlun Ju 1 , Yulin Fang 1
Affiliation  

The BRANCHED1 (BRC1) gene has been reported as a key regulator of shoot branching in many model plants. However, its role in the shoot branching process of grapevine has not been described. In this present study, we isolated VvBRC, a member of the Teosinte branched 1/cycloidea/proliferating cell factor 1 (TCP) transcription factor family, from grapevine (Vitis vinifera L.). The VvBRC protein was localized in the nucleus. RT-qPCR analysis showed that VvBRC was primarily expressed in grapevine prompt and latent buds and stems. Moreover, VvBRC expression in grapevine prompt buds was suppressed in response to decapitation, and induced in response to hormones such as cytokinin and gibberellin. Heterologous overexpression of VvBRC inhibited plant shoot branching in transgenic Arabidopsis, and complementary expression restored the phenotype in mutant Arabidopsis thaliana. In addition, transcript levels of genes participating in ABA biosynthesis, such as NCED, and ABA signaling pathways, such as ABF3, ABI5, HB21, HB40, and HB53, were upregulated, whereas the transcript levels of genes involved in the cell cycle, such as CYCD3 and HISTONE H4, were downregulated in VvBRC-overexpressing plants. Collectively, these results suggest that VvBRC play critical and negative roles in grapevine shoot branching.



中文翻译:

VvBRC 抑制葡萄树的枝条分枝

BRANCHED1BRC1)基因已被报告为拍摄的许多模式植物分支的一个关键调节器。然而,尚未描述其在葡萄枝条分枝过程中的作用。在本研究中,我们从葡萄 ( Vitis vinifera L.) 中分离了VvBRC,它是Teosinte分支 1/cycloidea/增殖细胞因子 1 (TCP) 转录因子家族的成员。VvBRC 蛋白位于细胞核中。RT-qPCR 分析表明VvBRC主要在葡萄树的提示和潜芽和茎中表达。此外,VvBRC葡萄树萌芽中的表达因断头而被抑制,并因细胞分裂素和赤霉素等激素而被诱导。VvBRC的异源过表达抑制了转基因拟南芥的植物枝条分枝,而互补表达恢复了突变体拟南芥的表型。此外,参与 ABA 生物合成的基因,如NCED和 ABA 信号通路,如ABF3、ABI5、HB21、HB40HB53的转录水平上调,而参与细胞周期的基因的转录水平,如作为CYCD3HISTONE H4,在VvBRC - 过表达植物。总的来说,这些结果表明VvBRC在葡萄枝条分枝中起着关键和负面的作用。

更新日期:2021-07-30
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