Due to the emergence of multidrug-resistant bacteria, bacteriophages have become a viable alternative in controlling bacterial growth or biofilm formation. Biofilm is formed by extracellular polymeric substances (EPS) and is one of the factors responsible for increasing bacterial resistance. Bacteriophages have been studied as a bacterial control agent by use of phage enzymes or due to their bactericidal activities. A specific phage against Serratia marcescens was isolated in this work and was evaluated its biological and genomic aspects. The object of this study was UFV01, a bacteriophage belonging to the Podoviridae family, genus Teseptimavirus (group of lytic viruses), specific to the species S. marcescens, which may be related to several amino acid substitutions in the virus tail fibers. Despite this high specificity, the phage reduced the biofilm formation of several Escherichia coli strains without infecting them. UFV01 presents a relationship with phages of the genus Teseptimavirus, although it does not infect any of the E. coli strains evaluated, as these others do. All the characteristics make the phage an interesting alternative in biofilm control in hospital environments since small breaks in the biofilm matrix can lead to a complete collapse.

由于多重耐药菌的出现，噬菌体已成为控制细菌生长或生物膜形成的可行替代方案。生物膜由细胞外聚合物(EPS) 形成，是导致细菌耐药性增加的因素之一。已经通过使用噬菌体酶或由于它们的杀菌活性研究了噬菌体作为细菌控制剂。在这项工作中分离了一种针对粘质沙雷氏菌的特定噬菌体，并对其生物学和基因组方面进行了评估。本研究的对象是 UFV01，一种噬菌体，属于 Podoviridae 家族，Teseptimavirus 属（裂解病毒组），特定于S. marcescens，这可能与病毒尾纤维中的几个氨基酸取代有关。尽管具有如此高的特异性，但噬菌体减少了几种大肠杆菌菌株的生物膜形成而不会感染它们。UFV01 与 Teseptimavirus 属的噬菌体存在关系，尽管它不像其他大肠杆菌菌株那样感染任何被评估的大肠杆菌菌株。所有这些特性使噬菌体成为医院环境中生物膜控制的有趣替代方案，因为生物膜基质中的小破裂会导致完全崩溃。