The optimization and kinetic modeling of curdlan synthesis by Agrobacterium radiobacter mutant A-15 under multi-physiological-parameters process control, especially employing the on-line oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER) were performed in a 50-L fermentor. Results indicated that the maximum production of curdlan reach about 32.5 g/L with a glucose conversion yield of 49.5%, the maximum specific growth rate was about 0.5338 h⁻¹, the kinetics equation were of positive significance for production. It also demonstrated that A-15 was insensitive to catabolite repression during polysaccharide production for its maintaining high conversion yield in the medium contained 6.6% (W/V) initial glucose. In addition, the OUR and CER at the stationary growth stage showed a positive correlation with curdlan synthesis. This work will provide more theoretical support for large-scale curdlan production.

由优化和凝胶多糖合成的动力学模型放射形土壤杆菌突变体A-15下的多生理参数过程控制，特别是采用在线耗氧速率（OUR）和一个50进行二氧化碳释放速率（CER） L 发酵罐。结果表明，凝胶多糖的最大产量约为32.5 g/L，葡萄糖转化率为49.5%，最大比增长率约为0.5338 h ^-1，动力学方程对生产具有积极意义。它还表明，A-15 在多糖生产过程中对分解代谢物的抑制不敏感，因为它在含有 6.6% (W/V) 初始葡萄糖的培养基中保持高转化率。此外，稳定生长期的OUR和CER与凝胶多糖的合成呈正相关。该工作将为凝胶多糖的规模化生产提供更多的理论支持。