Isolated microspore culture (IMC) is a powerful single cell system with many advantages compared with other culturing methods for Brassica species. For nearly 40 years, numerous studies have explored the influencing factors on the isolated microspore process, the ability of microspore-derived embryos (MDEs) to germinate and develop into plantlets, and the process of plant regeneration in Brassica species. In this review, the influencing factors, changes in gene expression, and the physiological and biochemical changes during the procedure are discussed at length. Ultimately, this review presents a standard protocol to improve the efficiency of IMC in Brassica haploid and double haploid (DH) production technologies.

与其他芸苔属植物培养方法相比，分离小孢子培养 (IMC) 是一种强大的单细胞系统，具有许多优势。近40年来，大量研究探讨了影响小孢子分离过程、小孢子衍生胚（MDEs）萌发和发育成小苗的能力以及芸苔属植物再生过程的影响因素。在这篇综述中，对影响因素、基因表达的变化以及手术过程中的生理生化变化进行了详细讨论。最后，这篇综述提出了一个标准协议，以提高 IMC 在芸苔属单倍体和双单倍体 (DH) 生产技术中的效率。