Abstract

Calliandra portoricensis (C. portoricensis) is used in herbal homes in Nigeria to manage breast diseases. We investigated the anti-tumourigenic effects of chloroform extract of C. portoricensis (CP) in breast experimental cancer induced by N-methyl-N-nitrosourea (NMU) and benzo-(a)-pyrene (BaP). Fifty-six female rats were assigned into seven equal groups: Group 1 served as control, group 2 received NMU and BaP (50 mg/kg, each), groups 3 and 4 received [NMU + BaP] and treated with CP at 50 and 100 mg/kg, respectively. Group 5 received CP (100 mg/kg), group 6 received [NMU + BaP] and vincristine (0.5 mg/kg), while group 7 received vincristine (0.5 mg/kg). The NMU and BaP (i.p) were dissolved in normal saline and corn oil, respectively. The CP (oral) and vincristine (i.p) were given thrice and twice per week, respectively for 10 weeks. The [NMU + BaP] intoxication significantly decreased body weight gain by 32% while organo-somatic weight of mammary gland increased by 37%. Also, [NMU + BaP] decreased the activities of mammary catalase, glutathione-s-transferase, glutathione peroxidase, superoxide dismutase and total sulphurhydryl by 34%, 31%, 35%, 35% and 33%, respectively. The [NMU + BaP] increased inflammatory and oxidative stress markers; nitrite, lipid peroxidation and myeloperoxidase by 62%, 57% and 361%, respectively. Strong expression of BCL-2, IL-6, COX 2, β-catenin and iNOS in [NMU + BaP]-administered rats were observed. Histology revealed glands with malignant epithelial cells and high nucleocytoplasm in [NMU + BaP] rats. Treatment with CP attenuated inflammation, apoptosis and restored cyto-architecture of mammary gland. Overall, CP abates mammary tumourigenesis by targeting cellular pathways of inflammation and apoptosis.

摘要

Calliandra portoricensis ( C. portoricensis ) 在尼日利亚的草药屋中用于治疗乳腺疾病。我们研究了C. portoricensis (CP) 氯仿提取物在N-甲基-N-亚硝基脲 (NMU) 和苯并-(a)-芘 (BaP)诱导的乳腺癌实验中的抗肿瘤作用。将 56 只雌性大鼠分成 7 个相等的组：第 1 组作为对照组，第 2 组接受 NMU 和 BaP（ 各 50 mg/kg），第 3 组和第 4 组接受 [NMU + BaP] 并在 50 和 CP 治疗分别为100 毫克/千克。第 5 组收到 CP (100 mg/kg)，第 6 组接受 [NMU + BaP] 和长春新碱 (0.5 mg/kg)，而第 7 组接受长春新碱 (0.5 mg/kg)。NMU 和 BaP (ip) 分别溶解在生理盐水和玉米油中。CP（口服）和长春新碱（ip）分别每周给予三次和两次，持续 10 周。[NMU + BaP] 中毒显着降低了 32% 的体重增加，而乳腺的有机体重量增加了 37%。此外，[NMU + BaP] 使乳腺过氧化氢酶、谷胱甘肽-s-转移酶、谷胱甘肽过氧化物酶、超氧化物歧化酶和总巯基的活性分别降低了 34%、31%、35%、35% 和 33%。[NMU + BaP] 增加炎症和氧化应激标志物；亚硝酸盐、脂质过氧化和髓过氧化物酶分别提高了 62%、57% 和 361%。BCL-2、IL-6、COX 2、在 [NMU + BaP] 给药的大鼠中观察到 β-连环蛋白和 iNOS。组织学显示 [NMU + BaP] 大鼠腺体具有恶性上皮细胞和高核质。用 CP 治疗可减轻炎症、细胞凋亡并恢复乳腺的细胞结构。总体而言，CP 通过靶向炎症和细胞凋亡的细胞途径来减轻乳腺肿瘤的发生。