Natural DNA is exquisitely evolved to store genetic information. The chirally inverted l-DNA, possessing the same informational capacity but resistant to biodegradation, may serve as a robust, bioorthogonal information repository. Here we chemically synthesize a 90-kDa high-fidelity mirror-image Pfu DNA polymerase that enables accurate assembly of a kilobase-sized mirror-image gene. We use the polymerase to encode in l-DNA an 1860 paragraph by Louis Pasteur that first proposed a mirror-image world of biology. We realize chiral steganography by embedding a chimeric d-DNA/l-DNA key molecule in a d-DNA storage library, which conveys a false or secret message depending on the chirality of reading. Furthermore, we show that a trace amount of an l-DNA barcode preserved in water from a local pond remains amplifiable and sequenceable for 1 year, whereas a d-DNA barcode under the same conditions could not be amplified after 1 day. These next-generation mirror-image molecular tools may transform the development of advanced mirror-image biology systems and pave the way for the realization of the mirror-image central dogma and exploration of their applications.

天然DNA经过精巧进化以存储遗传信息。手性倒置的l- DNA 具有相同的信息容量但抗生物降解，可以作为一个强大的生物正交信息库。在这里，我们化学合成了一种 90-kDa 高保真镜像Pfu DNA 聚合酶，能够准确组装千碱基大小的镜像基因。我们使用聚合酶在l- DNA 中编码 Louis Pasteur 1860 年首次提出生物学镜像世界的段落。我们通过在d中嵌入嵌合d -DNA/ l -DNA 关键分子来实现手性隐写术-DNA存储库，根据阅读的手性传达虚假或秘密信息。此外，我们表明，保存在当地池塘水中的微量l- DNA 条形码在 1 年内仍可扩增和测序，而在相同条件下的d - DNA 条形码在 1 天后无法扩增。这些下一代镜像分子工具可能会改变先进镜像生物学系统的发展，并为镜像中心法则的实现和应用探索铺平道路。