While embryonic stem cells and cancer cells are known to have many similarities in signalling pathways, healthy somatic cells are known to be different in many ways. Characterization of embryonic stem cell is crucial for cancer development and cancer recurrence due to the shared signalling pathways and life course with cancer initiator and cancer stem cells. Since embryonic stem cells are the sources of the somatic and cancer cells, it is necessary to reveal the relevance between them. The past decade has seen the importance of interdisciplinary studies and it is obvious that the reflection of the physical/chemical phenomena occurring on the cell biology has attracted much more attention. For this reason, the aim of this study is to elementally and topologically characterize the mouse embryonic stem cells, mouse lung squamous cancer cells, and mouse skin fibroblast cells by using Atomic Force Microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and Scanning Electron Microscopy (SEM) supported with Electron Dispersive Spectroscopy (EDS) techniques in a complementary way. Our AFM findings revealed that roughness data of the mouse embryonic stem cells and cancer cells were similar and somatic cells were found to be statistically different from these two cell types. However, based on both XPS and SEM-EDS results, surface elemental ratios vary in mouse embryonic stem cells, cancer cells and somatic cells. Our results showed that these complementary spectroscopic and microscopic techniques used in this work are very effective in cancer and stem cell characterization and have the potential to gather more detailed information on relevant biological samples.

虽然已知胚胎干细胞和癌细胞在信号通路上有许多相似之处，但已知健康体细胞在许多方面都不同。由于与癌症起始者和癌症干细胞共享信号通路和生命过程，胚胎干细胞的表征对于癌症发展和癌症复发至关重要。由于胚胎干细胞是体细胞和癌细胞的来源，因此有必要揭示它们之间的相关性。过去十年见证了跨学科研究的重要性，很明显，对细胞生物学上发生的物理/化学现象的反映引起了更多的关注。因此，本研究的目的是从元素和拓扑上表征小鼠胚胎干细胞、小鼠肺鳞状细胞、通过使用原子力显微镜 (AFM)、X 射线光电子能谱 (XPS) 和扫描电子显微镜 (SEM) 以互补的方式支持电子色散光谱 (EDS) 技术和小鼠皮肤成纤维细胞。我们的 AFM 研究结果表明，小鼠胚胎干细胞和癌细胞的粗糙度数据相似，并且发现体细胞在统计学上与这两种细胞类型不同。然而，基于 XPS 和 SEM-EDS 结果，小鼠胚胎干细胞、癌细胞和体细胞的表面元素比率不同。我们的研究结果表明，这项工作中使用的这些互补的光谱和显微技术在癌症和干细胞表征方面非常有效，并且有可能收集有关相关生物样本的更详细信息。