Congenital tremor (CT) type A-II in piglets is a worldwide disease caused by an emerging atypical porcine pestivirus (APPV). Preparation and evaluation of vaccines in laboratory animals is an important preliminary step toward prevention and control of the disease. Here, virus-like particles (VLPs) of APPV were prepared and VLPs vaccine was evaluated in BALB/c mice. Purified E^rns and E2 proteins expressed in E. coli were allowed to self-assemble into VLPs, which had the appearance of hollow spherical particles with a diameter of about 100 nm by transmission electron microscopy (TEM). The VLPs induced strong antibody responses and reduced the viral load in tissues of BALB/c mice. The data from animal challenge experiments, RT-PCR, and immunohistochemical analysis demonstrated that BALB/c mice are an appropriate laboratory model for APPV. These results suggest the feasibility of using VLPs as a vaccine for the prevention and control of APPV and provide useful information for further study of APPV in laboratory animals.

仔猪先天性震颤 (CT) A-II 型是一种由新出现的非典型猪瘟病毒 (APPV) 引起的全球性疾病。在实验动物身上制备和评估疫苗是预防和控制该疾病的重要一步。在这里，制备了 APPV 的病毒样颗粒 (VLPs)，并在 BALB/c 小鼠中评估了 VLPs 疫苗。在大肠杆菌中表达的纯化的^Erns和 E2 蛋白允许自组装成 VLP，通过透射电子显微镜（TEM），其外观为直径约 100 nm 的中空球形颗粒。VLPs 诱导强烈的抗体反应并降低 BALB/c 小鼠组织中的病毒载量。来自动物攻击实验、RT-PCR 和免疫组织化学分析的数据表明，BALB/c 小鼠是 APPV 的合适实验室模型。这些结果表明使用 VLP 作为疫苗预防和控制 APPV 的可行性，并为进一步研究实验室动物中的 APPV 提供了有用的信息。