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Rapid Characterization of Proteinaceous Binders Used in Artwork and Cultural Heritage Materials by Surface-Enhanced Raman Spectroscopy (SERS)
Analytical Letters ( IF 2 ) Pub Date : 2021-07-29 , DOI: 10.1080/00032719.2021.1948049
Yujie He 1 , Ming Wen 1 , Xiao Zhou 2 , Feng Gao 2 , Hongmei Lu 1
Affiliation  

Abstract

The identification and characterization of binders in art and cultural heritage materials such as manuscripts, drawings, and paintings provide important information for dating, authentication, and conservation. However, the common methods are usually invasive and require micro-sampling. Here, a simple and rapid method was established based on surface-enhanced Raman spectroscopy (SERS) for quasi-uninvasive determination of proteinaceous binders with a portable Raman spectrometer. Initially, silica sol was prepared via a one-step synthesis and deposited on the germination paper to obtain a silica film. After being cut and wetted, 5-mm × 5-mm pieces of silica film were attached to the surface of the test object. The proteinaceous binders were extracted from the test objects with the interaction between silica, followed by SERS analysis using silver nanoparticles as the substrate. Scanning electron microscopic and optical microscopic images confirm that the surface of the samples was uninvasively damaged after extraction. The feasibility and reproducibility of this method were confirmed using paint models. The minimum detectable concentration of bovine serum albumin in the paint models was 0.005% (w/w). The method was successfully applied to determine the proteinaceous binders in fresh or aged paint replicas. Due to advantages that include rapid, quasi-non-invasive, and on-site analysis, this protocol is promising for the determination of proteinaceous binders employed in culture heritage materials.



中文翻译:

通过表面增强拉曼光谱 (SERS) 快速表征艺术品和文化遗产材料中使用的蛋白质粘合剂

摘要

手稿、素描和绘画等艺术和文化遗产材料中的粘合剂的识别和表征为约会、鉴定和保护提供了重要信息。然而,常用的方法通常是侵入性的,需要微量采样。在这里,建立了一种基于表面增强拉曼光谱 (SERS) 的简单快速的方法,用于使用便携式拉曼光谱仪准无创测定蛋白质粘合剂。最初,硅溶胶是通过一步合成并沉积在发芽纸上,得到二氧化硅薄膜。切割并润湿后,将 5-mm × 5-mm 的二氧化硅薄膜贴在测试对象的表面。通过二氧化硅之间的相互作用从测试对象中提取蛋白质粘合剂,然后使用银纳米粒子作为基底进行 SERS 分析。扫描电子显微镜和光学显微镜图像证实,样品表面在提取后受到无创损伤。使用油漆模型证实了该方法的可行性和可重复性。油漆模型中牛血清白蛋白的最低可检测浓度为 0.005% (w/w)。该方法成功地应用于测定新鲜或老化油漆复制品中的蛋白质粘合剂。由于包括快速、

更新日期:2021-07-29
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