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Use of hCG hormone in the natural and artificial reproduction of Nile tilapia (Oreochromis niloticus)
Aquaculture Research ( IF 2 ) Pub Date : 2021-07-29 , DOI: 10.1111/are.15502
Ragli Oliveira Azevedo 1 , Érika Ramos Alvarenga 1 , Arthur Francisco Araújo Fernandes 2 , Marcos Antonio Silva 1 , Gabriel Francisco de Oliveira Alves 1 , Williane Ferreira Menezes 1 , Eduardo Maldonado Turra 1
Affiliation  

This study aimed to develop a protocol to synchronize final oocyte maturation and oocyte collection in both natural (first experiment) and artificial reproduction (second experiment) of Nile tilapia. In the first experiment, 80 young females (1 year old), 40 old females (3 years old) and 75 young males were used and splitted into three different groups. Each group bred once by natural mating during 7 days. Each female received an injection of hCG (3 IU/g of female) or saline solution (control) just before being stocked with males. In the second experiment, we evaluated different doses (0.5, 1.5, 2.5 and 3.5 IU/g female), number of injections (1 or 2) and administration route (intracoelomic or intramuscular) of hCG hormone in 40 females for artificial reproduction by stripping. The use of hCG (3 IU/g of female) was not effective for synchronization of natural reproduction in Nile tilapia, and the negative effect of age on reproductive performance was not reversed by its use. In the second assay, the use of a single injection with lower doses of hCG (0.5 and 1.5 IU/g of female) resulted in 100% of spawned females in artificial reproduction.

中文翻译:

hCG 激素在尼罗罗非鱼 (Oreochromis niloticus) 的自然和人工繁殖中的应用

本研究旨在开发一种方案,以同步尼罗罗非鱼的自然(第一次实验)和人工繁殖(第二次实验)中的最终卵母细胞成熟和卵母细胞收集。在第一个实验中,使用了80名年轻女性(1岁)、40名老年女性(3岁)和75名年轻男性并分成三个不同的组。每组在 7 天内通过自然交配繁殖一次。每只雌性在放养雄性之前都接受了 hCG(3 IU/g 雌性)或生理盐水(对照)的注射。在第二个实验中,我们评估了 40 只雌性的 hCG 激素在不同剂量(0.5、1.5、2.5 和 3.5 IU/g 雌性)、注射次数(1 或 2 次)和给药途径(腔内或肌内)的情况下通过剥脱进行人工繁殖. 使用 hCG(3 IU/g 雌性)对尼罗罗非鱼的自然繁殖同步无效,并且年龄对繁殖性能的负面影响并没有被其使用逆转。在第二个试验中,使用较低剂量的 hCG(0.5 和 1.5 IU/g 雌性)单次注射导致 100% 的雌性在人工繁殖中产卵。
更新日期:2021-07-29
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