Objective

To study the effect and mechanism of Schistosoma japonicum soluble egg antigen (SEA) on protecting against type 1 diabetes, 4-week-old female BALB/c and NOD mice were divided randomly into four groups: BALB/c control, BALB/c treated with SEA, NOD control, and NOD treated with SEA.

Methods

Treated mice were injected intraperitoneally with 50 μg of SEA twice a week for 6 weeks, and control mice received the same volume of phosphate-buffered saline. Blood glucose in all mice was determined weekly from 8 weeks of age. Flow cytometry was used to detect the percentages of regulatory T cells of splenocytes in each group. Enzyme-linked immunosorbent assays were used to detect the levels of interferon-γ, interleukin (IL)-2, IL-4, and IL-5 in splenic cell culture supernatants.

Results

Compared with those of the NOD group, the blood glucose level and percentage incidence of diabetes in NOD mice treated with SEA decreased significantly. This indicated that SEA treatment prevented spontaneous type 1 diabetes. After SEA administration, the frequency of splenic regulatory T cells increased significantly, and the secretion of IL-4 and IL-5 by splenic cells increased.

Conclusions

These results demonstrated that SEA can prevent type 1 diabetes by enhancing regulatory T cells and the T helper 2 cell immune response in NOD mice.

中文翻译：

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日本血吸虫可溶性卵抗原对NOD小鼠1型糖尿病的保护作用及机制

客观的

为研究日本血吸虫可溶性卵抗原 (SEA) 对 1 型糖尿病的保护作用及其机制，将 4 周龄雌性 BALB/c 和 NOD 小鼠随机分为四组：BALB/c 对照组、BALB/c 治疗组用 SEA、NOD 对照和用 SEA 处理的 NOD。

方法

接受治疗的小鼠每周两次腹膜内注射 50 μg SEA，持续 6 周，对照组小鼠接受相同体积的磷酸盐缓冲盐水。从 8 周龄开始每周测定所有小鼠的血糖。流式细胞术检测各组脾细胞调节性T细胞百分比。酶联免疫吸附试验用于检测脾细胞培养上清液中干扰素-γ、白细胞介素 (IL)-2、IL-4 和 IL-5 的水平。

结果

与NOD组相比，经SEA处理的NOD小鼠的血糖水平和糖尿病发病率均显着降低。这表明 SEA 治疗预防了自发性 1 型糖尿病。SEA给药后，脾调节性T细胞的频率显着增加，脾细胞分泌IL-4和IL-5增加。

结论

这些结果表明，SEA 可以通过增强 NOD 小鼠的调节性 T 细胞和 T 辅助 2 细胞免疫反应来预防 1 型糖尿病。