Abstract

A possibility of replacing acetonitrile for methanol in mobile phases containing an organic modifier, 10 vol. % of formic acid (pH regulator), and water in the separation of anthocyanins by reversed-phase HPLC on a C18 stationary phase (Symmetry^®C18) is studied. It is found that in replacing acetonitrile for methanol, it is necessary to use two components of the mobile phase, containing only methanol and only formic acid, as on the joint presence of these substances, a prolonged retention drift is observed, presumably because of the acylation of methanol. It is found that the replacement of acetonitrile for methanol results in small changes in the selectivity of the separation of anthocyanins with identical substituents but different aglycones and with different carbohydrate substituents for one and the same aglycone. There are also no significant differences in the separation efficiency (by the number of theoretical plates) of anthocyanins, which makes it possible to reject expensive acetonitrile in favor of methanol. The developed procedure was applied to determine anthocyanins in cranberry fruits of various degrees of ripeness (by color). The specific features of the anthocyanin composition of cranberry fruits are determined and the need in controlling the composition of the sample solvent for excluding artifact peaks is shown.

中文翻译：

---

花青素分离的选择性控制：用乙腈代替流动相中的甲醇

摘要

在含有有机改性剂的流动相中用乙腈代替甲醇的可能性，10 体积。通过反相 HPLC 在 C18 固定相 (Symmetry ^®C18) 进行了研究。发现在用乙腈代替甲醇时，必须使用两种流动相组分，仅含甲醇和仅含甲酸，因为在这些物质的共同存在下，观察到延长的保留漂移，大概是因为甲醇酰化。发现用乙腈代替甲醇导致分离具有相同取代基但苷元不同的花青苷以及对一个和相同苷元具有不同碳水化合物取代基的花青苷的选择性变化很小。花青素的分离效率（理论塔板数）也没有显着差异，这使得有可能拒绝昂贵的乙腈而有利于甲醇。所开发的程序用于测定不同成熟度（按颜色）的蔓越莓果实中的花青素。确定了蔓越莓果实花青素成分的具体特征，并显示了控制样品溶剂成分以排除伪峰的必要性。