We report on a simple, fluorescence-based method for the investigation of the binding kinetics of polystyrene ligands, dispersed in an organic solvent, to substrate supported gold nanoparticles. For this purpose, we develop a protocol for the immobilization of gold nanoparticles on glass substrates, that yields sub-monolayers of randomly distributed particles with excellent homogeneity and reproducibility. Using fluorescently labeled polystyrene, we monitor the ligand concentration in bulk dispersion in real time and follow the binding to the particle-decorated substrates. The influence of the ligand molecular weight on the binding kinetics is investigated. We correlate the reaction rates with the diffusion coefficients of the different ligands and are able to describe the molecular weight dependency with a simple kinetic model. Both the diffusion and the activation step appear to contribute to the effective reaction rates.

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聚合物配体与表面固定化金纳米粒子的结合：基于荧光的吸附动力学研究

我们报告了一种简单的、基于荧光的方法，用于研究分散在有机溶剂中的聚苯乙烯配体与基底支持的金纳米粒子的结合动力学。为此，我们开发了一种将金纳米粒子固定在玻璃基板上的协议，该协议产生了具有优异均匀性和重现性的随机分布粒子的亚单层。使用荧光标记的聚苯乙烯，我们实时监测散装分散体中的配体浓度，并跟踪与颗粒装饰基板的结合。研究了配体分子量对结合动力学的影响。我们将反应速率与不同配体的扩散系数相关联，并且能够用简单的动力学模型描述分子量依赖性。