About 10% of human proteins have no annotated function in protein knowledge bases. A workflow to generate hypotheses for the function of these uncharacterized proteins has been developed, based on predicted and experimental information on protein properties, interactions, tissular expression, subcellular localization, conservation in other organisms, as well as phenotypic data in mutant model organisms. This workflow has been applied to seven uncharacterized human proteins (C6orf118, C7orf25, CXorf58, RSRP1, SMLR1, TMEM53 and TMEM232) in the frame of a course-based undergraduate research experience named Functionathon organized at the University of Geneva to teach undergraduate students how to use biological databases and bioinformatics tools and interpret the results. C6orf118, CXorf58 and TMEM232 were proposed to be involved in cilia-related functions; TMEM53 and SMLR1 were proposed to be involved in lipid metabolism and C7orf25 and RSRP1 were proposed to be involved in RNA metabolism and gene expression. Experimental strategies to test these hypotheses were also discussed. The results of this manual data mining study may contribute to the project recently launched by the Human Proteome Organization (HUPO) Human Proteome Project aiming to fill gaps in the functional annotation of human proteins. Database URL: http://www.nextprot.org

中文翻译：

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Functionathon：一种手动数据挖掘工作流程，用于为未表征的人类蛋白质生成功能假设及其在本科生中的应用

大约 10% 的人类蛋白质在蛋白质知识库中没有注释功能。基于蛋白质特性、相互作用、组织表达、亚细胞定位、其他生物体中的保守性以及突变模型生物体中的表型数据的预测和实验信息，已经开发了一种工作流程来生成这些未表征蛋白质的功能假设。在日内瓦大学组织的名为 Functionathon 的基于课程的本科研究体验框架中，该工作流程已应用于七种未表征的人类蛋白质（C6orf118、C7orf25、CXorf58、RSRP1、SMLR1、TMEM53 和 TMEM232），以教授本科生如何使用生物数据库和生物信息学工具并解释结果。C6orf118, CXorf58 和 TMEM232 被提议参与纤毛相关功能；TMEM53 和 SMLR1 被提议参与脂质代谢，而 C7orf25 和 RSRP1 被提议参与 RNA 代谢和基因表达。还讨论了检验这些假设的实验策略。这项手动数据挖掘研究的结果可能有助于人类蛋白质组组织 (HUPO) 人类蛋白质组计划最近启动的项目，旨在填补人类蛋白质功能注释方面的空白。数据库网址：http://www.nextprot.org 这项手动数据挖掘研究的结果可能有助于人类蛋白质组组织 (HUPO) 人类蛋白质组计划最近启动的项目，旨在填补人类蛋白质功能注释方面的空白。数据库网址：http://www.nextprot.org 这项手动数据挖掘研究的结果可能有助于人类蛋白质组组织 (HUPO) 人类蛋白质组计划最近启动的项目，旨在填补人类蛋白质功能注释方面的空白。数据库网址：http://www.nextprot.org