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Dynamic changes in RNA-protein interactions and RNA secondary structure in mammalian erythropoiesis.
Life Science Alliance ( IF 4.4 ) Pub Date : 2021-07-27 , DOI: 10.26508/lsa.202000659
Mengge Shan 1, 2 , Xinjun Ji 3 , Kevin Janssen 4 , Ian M Silverman 3 , Jesse Humenik 3 , Ben A Garcia 4 , Stephen A Liebhaber 5, 6 , Brian D Gregory 2, 7
Affiliation  

Two features of eukaryotic RNA molecules that regulate their post-transcriptional fates are RNA secondary structure and RNA-binding protein (RBP) interaction sites. However, a comprehensive global overview of the dynamic nature of these sequence features during erythropoiesis has never been obtained. Here, we use our ribonuclease-mediated structure and RBP-binding site mapping approach to reveal the global landscape of RNA secondary structure and RBP-RNA interaction sites and the dynamics of these features during this important developmental process. We identify dynamic patterns of RNA secondary structure and RBP binding throughout the process and determine a set of corresponding protein-bound sequence motifs along with their dynamic structural and RBP-binding contexts. Finally, using these dynamically bound sequences, we identify a number of RBPs that have known and putative key functions in post-transcriptional regulation during mammalian erythropoiesis. In total, this global analysis reveals new post-transcriptional regulators of mammalian blood cell development.

中文翻译:

哺乳动物红细胞生成中 RNA-蛋白质相互作用和 RNA 二级结构的动态变化。

调节其转录后命运的真核 RNA 分子的两个特征是 RNA 二级结构和 RNA 结合蛋白 (RBP) 相互作用位点。然而,从未获得过关于红细胞生成过程中这些序列特征的动态性质的全面全局概述。在这里,我们使用我们的核糖核酸酶介导的结构和 RBP 结合位点作图方法来揭示 RNA 二级结构和 RBP-RNA 相互作用位点的全球景观以及这些特征在这一重要发育过程中的动态。我们在整个过程中识别 RNA 二级结构和 RBP 结合的动态模式,并确定一组相应的蛋白质结合序列基序及其动态结构和 RBP 结合上下文。最后,使用这些动态绑定的序列,我们确定了许多在哺乳动物红细胞生成过程中在转录后调控中具有已知和推定关键功能的 RBP。总的来说,这项全球分析揭示了哺乳动物血细胞发育的新转录后调节因子。
更新日期:2021-07-27
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