Background. KLF4 and KLF5 are members of the KLF transcription factor family, which play an important role in many gastrointestinal tumors. To gain a deeper insight into its function and role, bioinformatics was used to analyze the function and role of KLF4 and KLF5 in gastrointestinal tumors. Methods. Data were collected from several online databases. Gene Expression Profiling Interactive Analysis (GEPIA), UALCAN database analysis, Kaplan-Meier Plotter analysis, LOGpc system, the Pathology Atlas, and the STRING website were used to analyze the data. We download relevant data from TCGA and then perform GO enrichment and KEGG enrichment analysis. The effects of KLF5 on gastric cancer cell proliferation were measured by CCK-8 assay. The effect of KLF5 on the expression of CyclinD1 and MMP9 was detected by Western blot. Results. KLF4 and KLF5 were differentially expressed in normal and tumor tissues of the gastrointestinal tract, and their differential expression is related to several genes or pathways. KEGG analysis showed that KLF5 was coexpressed with endocytosis-related genes. KLF5 promotes the proliferation of gastric cancer cells and the expression of metastasis-related molecules. Conclusion. KLF4 and KLF5 are of great significance for developing gastrointestinal tumors and can be used as therapeutic targets.

中文翻译：

---

KLF4 和 KLF5 在胃肠道肿瘤中的部分作用

背景。KLF4和KLF5是KLF转录因子家族的成员，在许多胃肠道肿瘤中起重要作用。为了更深入地了解其功能和作用，生物信息学被用来分析KLF4和KLF5在胃肠道肿瘤中的功能和作用。方法. 数据是从几个在线数据库中收集的。基因表达谱交互分析（GEPIA）、UALCAN 数据库分析、Kaplan-Meier Plotter 分析、LOGpc 系统、病理学图谱和 STRING 网站用于分析数据。我们从 TCGA 下载相关数据，然后进行 GO 富集和 KEGG 富集分析。采用CCK-8法测定KLF5对胃癌细胞增殖的影响。Western blot检测KLF5对CyclinD1和MMP9表达的影响。结果. KLF4和KLF5在胃肠道正常组织和肿瘤组织中均有差异表达，其差异表达与多个基因或通路有关。KEGG分析显示KLF5与内吞相关基因共表达。KLF5促进胃癌细胞的增殖和转移相关分子的表达。结论。KLF4和KLF5对于发展胃肠道肿瘤具有重要意义，可作为治疗靶点。