Ag nanoparticles and Ag/GO nanocomposites have been synthesized by facile, eco-friendly, and cost-effective green approach using the peel extracts of Punica granatum. The synthesized Silver Nanoparticles and Silver Graphene Oxide nanocomposites were characterized by UV–visible spectroscopy, X-ray diffraction, transmission electron microscopy, and Fourier transform infrared analysis. AgNPs revealed spherical morphology with particle size ranging from 30 to 40 nm. Scanning electron microscopy results revealed silver nanoparticles with an average size of 35 nm being uniformly distributed over GO sheets. The surface plasmon band appears at 433 nm for AgNPs and 458 nm for Ag/GO nanocomposites. The green synthesized nanoparticles were found to have excellent antibacterial efficacy against both gram positive (Staphylococcus aureus, Bacillus subtilis) and gram negative (Klebsiella pneumoniae, Escherichia coli) bacterial strains. The results showed that Ag/GO nanocomposites showed better antibacterial activity than pure AgNPs with maximum inhibitory zone of 28 mm for Escherichia coli. This attributes to the role of GO sheets in preventing AgNPs from agglomerating and oxidizing. The anticancer activity of AgNPs and Ag/GO nanocomposites were tested against human breast cancer cell line (MCF-7) which demonstrated a dose dependent activity with half minimum inhibitory concentration of 100 and 150 μg/ml.

中文翻译：

---

阐明石榴皮提取物介导的 Ag 和 Ag/GO 纳米复合材料的结构、抗癌和抗菌特性

使用石榴果皮提取物，通过简便、环保且具有成本效益的绿色方法合成了 Ag 纳米粒子和 Ag/GO 纳米复合材料。合成的银纳米颗粒和银氧化石墨烯纳米复合材料通过紫外-可见光谱、X 射线衍射、透射电子显微镜和傅里叶变换红外分析进行表征。AgNPs 显示球形形态，粒径范围为 30 至 40 nm。扫描电子显微镜结果显示平均尺寸为 35 nm 的银纳米粒子均匀分布在 GO 片上。AgNPs 的表面等离子体带出现在 433 nm，Ag/GO 纳米复合材料出现在 458 nm。发现绿色合成纳米粒子对革兰氏阳性菌（金黄色葡萄球菌、枯草芽孢杆菌）和革兰氏阴性菌（肺炎克雷伯菌、大肠杆菌）菌株。结果表明，Ag/GO纳米复合材料比纯AgNPs表现出更好的抗菌活性，对大肠杆菌的最大抑菌圈为28 mm 。这归因于 GO 片在防止 AgNPs 团聚和氧化方面的作用。AgNPs 和 Ag/GO 纳米复合材料的抗癌活性针对人乳腺癌细胞系 (MCF-7) 进行了测试，MCF-7 表现出剂量依赖性活性，半数最小抑制浓度为 100 和 150 μg/ml。