Introduction: Alzheimer’s disease is the most popular neurodegenerative disorder with no effective drugs to stop the progression. Zuoguiwan (ZGW), a traditional Chinese herbal medicine, has been applied in many diseases. Our study aimed to detect the function and mechanisms of ZGW in Alzheimer’s disease (AD). Methods: The rat models of AD were established by streptozotocin (STZ), and the function of ZGW on cognitive dysfunction was measured with the Morris water maze test. The concentration of pro-inflammatory mediators was accessed by enzyme-linked immunosorbent assay. The relative mRNA expression of ERβ was detected by real-time quantitative PCR. Results: The treatment with ZGW could suppress the cognitive impairment by the findings of escape latency and time spent in the target quadrant and the increased concentration of IL-1β, IL-6, and TNF-α induced by STZ. STZ might repress the mRNA levels of ERβ, and ZGW management weakened the declined mRNA expression of ERβ. ZGW might play a protective role in AD rats against the injury of STZ on cognition and neuro-inflammation by improving the mRNA expression of ERβ. Conclusion: The results indicated that ZGW might be a novel therapeutic strategy to slow the process of AD by modulating ERβ.
Neuroimmunomodulation

中文翻译：

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左桂丸改善链脲佐菌素诱导的阿尔茨海默病大鼠的认知缺陷和神经炎症

简介：阿尔茨海默病是最流行的神经退行性疾病，没有有效的药物来阻止其进展。左桂丸（ZGW）是一种传统中草药，已应​​用于多种疾病。我们的研究旨在检测 ZGW 在阿尔茨海默病 (AD) 中的功能和机制。方法：采用链脲佐菌素（STZ）建立AD大鼠模型，采用Morris水迷宫法测定ZGW对认知功能障碍的影响。通过酶联免疫吸附测定获得促炎介质的浓度。通过实时定量PCR检测ERβ的相对mRNA表达。结果：ZGW 治疗可以通过逃避潜伏期和在目标象限所花费的时间以及 STZ 诱导的 IL-1β、IL-6 和 TNF-α 浓度增加来抑制认知障碍。STZ 可能抑制 ERβ 的 mRNA 水平，ZGW 管理减弱了 ERβ 的 mRNA 表达下降。ZGW可能通过改善ERβ的mRNA表达对AD大鼠的STZ对认知和神经炎症的损伤起到保护作用。结论：结果表明，ZGW 可能是一种通过调节 ERβ 来减缓 AD 进程的新治疗策略。
神经免疫调节