Introduction: Asymptomatic sensitization is defined as the presence of positive skin prick test (SPT) and/or positive serum allergen-specific IgE in the absence of clinical allergic symptoms. Currently, there is no convincing explanation why some people with positive allergen tests do not show symptoms. We aimed to investigate the house dust mite (HDM)-specific IgE and IgG4 repertoire in asymptomatic HDM-sensitized subjects and HDM-induced allergic rhinitis (AR) patients. Methods: A total of 48 subjects sensitized to HDM were included in this study: 27 had AR with/without asthma (symptomatic group), and 21 had no allergic symptoms (asymptomatic group). Six healthy individuals served as control group. Peripheral blood samples were collected for serum IgE and IgG4 assay and basophil activation tests (BATs). IgE and IgG4 assay included antibodies to Dermatophagoides (Der) p1, 2, 7, 10, 21, 23, and Der f1, 2. Results: AR patients had a larger wheal diameter of SPT (7.0 vs. 3.0 mm, p #x3c; 0.0001) and a higher specific IgE to Der p (15.50 vs. 0.70 KU/L, p #x3c; 0.0001) than asymptomatic subjects. They also showed more frequent sensitization to Der p1 and Der p2 (both p #x3c; 0.05). However, the total IgE and specific IgG4 did not differ significantly between the 2 groups. The basophil activation response after being stimulated with HDM was observed to be higher in AR patients (all p #x3c; 0.05). Conclusions: There are differences in SPT, serum-specific IgE to Der p, component allergen Der p1 and Der p2 level and BAT between AR patients and asymptomatic subjects sensitized to HDM. IgG4 alone cannot differentiate asymptomatic individuals from AR patients.
Int Arch Allergy Immunol

中文翻译：

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无症状 HDM 致敏和 HDM 诱发的过敏性鼻炎患者的 IgE 和 IgG4 库

简介：无症状致敏定义为在没有临床过敏症状的情况下，存在皮肤点刺试验 (SPT) 阳性和/或血清过敏原特异性 IgE 阳性。目前，没有令人信服的解释为什么有些过敏原检测呈阳性的人没有出现症状。我们旨在调查无症状的 HDM 致敏受试者和 HDM 诱发的过敏性鼻炎 (AR) 患者的屋尘螨 (HDM) 特异性 IgE 和 IgG4 库。方法：本研究共纳入 48 名对 HDM 敏感的受试者：27 名患有 AR 伴/不伴哮​​喘（症状组），21 人无过敏症状（无症状组）。六名健康人作为对照组。收集外周血样本用于血清 IgE 和 IgG4 测定以及嗜碱性粒细胞活化试验 (BAT)。IgE 和 IgG4 检测包括针对Dermatophagoides ( Der ) p1 , 2 , 7 , 10 , 21 , 23和Der f1 , 2 的抗体。结果： AR 患者的 SPT 风团直径较大（7.0 对 3.0 毫米，p#x3c; 0.0001）和比无症状受试者更高的Der p特异性 IgE （15.50 对 0.70 KU/L，p #x3c；0.0001）。他们还表现出对Der p1和Der p2更频繁的敏感性（均为p #x3c；0.05）。然而，两组之间的总 IgE 和特异性 IgG4 没有显着差异。在 AR 患者中观察到用 HDM 刺激后的嗜碱性粒细胞活化反应更高（所有p #x3c；0.05）。结论： SPT、血清特异性IgE对Der p、成分过敏原Der p1和Der p2存在差异AR患者和对HDM敏感的无症状受试者之间的水平和BAT。单独的 IgG4 不能区分无症状个体和 AR 患者。
Int Arch 过敏免疫