In this study, a TaqMan-based real-time polymerase chain reaction (PCR) method to detect canine astrovirus in clinical samples was developed. Primers and probes were designed to target conserved regions of the complete viral genome sequence. The results showed that the proposed method can detect a minimum of 10¹ copy numbers. No cross-reactivity with other canine and feline viruses was observed. The coefficient of variation was <5%. Evaluation of the clinical samples showed that quantitative PCR had a 5.26 % higher positive detection rate than conventional PCR. These results indicate that the method developed in this study is highly reliable and suitable for veterinary clinical diagnosis and epidemiological investigations.

在这项研究中，开发了一种基于 TaqMan 的实时聚合酶链反应 (PCR) 方法来检测临床样本中的犬星状病毒。引物和探针旨在靶向完整病毒基因组序列的保守区域。结果表明，所提出的方法可以检测到最少10 ^{1 个}拷贝数。未观察到与其他犬和猫病毒的交叉反应。变异系数<5%。对临床样本的评估表明，定量 PCR 的阳性检出率比常规 PCR 高 5.26%。这些结果表明，本研究中开发的方法高度可靠，适用于兽医临床诊断和流行病学调查。